Testing Data
Hamster anti-Mouse CD81C Antibody | anti-CD81C antibody
Hamster Anti-Mouse CD81-APC
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Synonyms
CD81C; Hamster Anti-Mouse CD81-APC; TAPA-1; anti-CD81C antibody
Host
Hamster
Reactivity
Mouse
Isotype
IgG3
Clone Number
2F7
Specificity
Mouse CD81
Form/Format
Supplied in PBS/NaN3 and a stabilizing agent
Concentration
0.1 mg/mL (varies by lot)
Applicable Applications for anti-CD81C antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Blocking (BL), Costimulation
Volume
1.0 mL
Immunogen
Mouse epithelial cell line PAM212
Conjugate
APC (Allophycocyanin)
Isotype Control
Hamster IgG-APC
Preparation and Storage
Store at 2-8 degree C.
Testing Data
Testing Data
Related Product Information for anti-CD81C antibody
CD81, also known as TAPA-1, is an integral membrane protein expressed on a variety of cell types and has a high degree of sequence homology between human and mouse. CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gammadelta+ T cells to more mature T cells with alphabeta T cell receptors. Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor which is composed of a TCR beta chain and glycoprotein pTalpha. The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro.
References
1. Boismenu R, Rhein M, Fischer WH, Havran WL. A role for CD81 in early T cell development. Science. 1996;271:198-200. (Immunogen, FC, IP, IHC, Block). 2. Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, et al. Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice. J Biol Chem. 2008;283:26089-97. (Block, Costim). 3. Tachibana I, Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J Cell Biol. 1999;146:893-904. (FC, Block). 4. Chang Y, Finnemann SC. Tetraspanin CD81 is required for the alphavbeta5-integrin-dependent particle-binding step of RPE phagocytosis. J Cell Sci. 2007;120:3053-63. (Block). 5. Keli S, Levy S, Suarez C, Weinstein DE. CD81 regulates neuron-induced astrocyte cell-cycle exit. Mol Cell Neurosci. 2001;17:551-60. (ICC)
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Product Notes
The CD81C (Catalog #AAA679284) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Anti-Mouse CD81-APC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD81C can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Blocking (BL), Costimulation. Researchers should empirically determine the suitability of the CD81C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD81C, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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