Rabbit anti-Human mtTFA Polyclonal Antibody | anti-TFAM antibody

Anti-mtTFA antibody

Cat. Num. AAA176374

• Gene Names: TFAM; TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: mtTFA; Polyclonal Antibody; Anti-mtTFA antibody; Transcription factor A; mitochondrial; transcription factor A; anscription factor 6-like 1 antibody; Mitochondrial transcription factor 1 antibody; mitochondrial transcription factor A antibody; MtTF1 antibody; mtTFA antibody; TCF 6 antibody; TCF-6 antibody; TCF6 antibody; TCF6L1 antibody; TCF6L2 antibody; TCF6L3 antibody; TFAM antibody; TFAM_HUMAN antibody; Transcription factor 6 antibody; Transcription factor 6 like 2(mitochondrial transcription factor) antibody; Transcription factor 6 like 2 antibody; Transcription factor 6-like 2 antibody; transcription factor 6-like 3 antibody; mitochondrial antibody; mitochondrial precursor antibody; anti-TFAM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 246

• Applicable Applications for anti-TFAM antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human mtTFA (227-246aa RKDLLRRTIKKQRKYGAEEC).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-mtTFA antibody, MBS176374, Western blottingLane 1: HELA Cell LysateLane 2: JURKAT Cell LysateLane 3: 293T Cell LysateLane 4: Rabbit igG(55kD)Lane 5: A431 Cell LysateLane 6: RAJI Cell LysateLane 7: CEM Cell LysateLane 8: HL-60 Cell LysateLane 9: HUT Cell Lysate )

Immunohistochemistry (IHC)

(Anti-mtTFA antibody, MBS176374, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunocytochemistry (ICC)

(Anti-mtTFA antibody, MBS176374, ICCICC: HELA Cell )

Immunohistochemistry (IHC)

(Anti-mtTFA antibody, MBS176374, IHC(F)IHC(F): Human Placenta Tissue )

Related Product Information for anti-TFAM antibody

Description: Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
Background: TFAM(Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA(mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al.(1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott(2007) stated that the sequences mapped to chromosomes 7p(TCF6L1) and 11q(MTTF1, or TCF6L3) are pseudogenes. Larsson et al.(1997) mapped the mouse mitochondrial transcription factor A gene(Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

NCBI and Uniprot Product Information

• NCBI GI #: 417324
• NCBI GeneID: 7019
• UniProt Accession #: Q00059; Q5U0C6; A8MRB2; A9QXC6; B5BU05
• Molecular Weight: 25,466 Da
• NCBI Official Full Name: Transcription factor A, mitochondrial
• NCBI Official Synonym Full Names: transcription factor A, mitochondrial
• NCBI Official Symbol: TFAM
• NCBI Official Synonym Symbols: TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
• NCBI Protein Information: transcription factor A, mitochondrial; transcription factor 6; transcription factor 6-like 1; transcription factor 6-like 3; mitochondrial transcription factor 1; mitochondrial transcription factor A; transcription factor 6-like 2 (mitochondrial transcription factor)
• UniProt Protein Name: Transcription factor A, mitochondrial
• Protein Family: Transcription factor
• UniProt Gene Name: TFAM
• UniProt Synonym Gene Names: TCF6; TCF6L2; mtTFA; MtTF1; TCF-6
• UniProt Entry Name: TFAM_HUMAN

NCBI Description

This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.

Protein type: DNA-binding; Transcription regulation

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: mitochondrion; mitochondrial matrix; cytosol; nucleus

Molecular Function: protein binding; heat shock protein binding; chromatin binding; transcription factor activity; DNA bending activity

Biological Process: chromatin remodeling; mitochondrial respiratory chain complex assembly; mitochondrion organization and biogenesis; regulation of transcription from RNA polymerase I promoter; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; gene expression; DNA-dependent DNA replication; transcription initiation from mitochondrial promoter; transcription from mitochondrial promoter