Western Blot (WB)
(Western Blot)
Mouse anti-Human XPF Monoclonal Antibody | anti-xpf antibody
XPF
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
XPF; Monoclonal Antibody; Anti -XPF; anti-xpf antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2,k
Clone Number
3F397
Specificity
Recognizes human XPF.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See X1049X.
Applicable Applications for anti-xpf antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Dilution: Western Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Immunogen
Recombinant human XPF protein. MW of Antigen: ~110kD. Cellular Localization: Nuclear.
Positive Control
MCF-7 cells or human tonsil.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot)
Western Blot (WB)
(Western Blot)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human tonsil stained with MBS601107 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphoid cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human tonsil stained with MBS601107 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphoid cells.)
Product Categories/Family for anti-xpf antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Xpf
NCBI Official Symbol
xpf
NCBI Protein Information
positive control sigma-like factor
Protein Family
Similar Products
Product Notes
The xpf (Catalog #AAA601107) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The XPF reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XPF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1-2ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Researchers should empirically determine the suitability of the xpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XPF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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