Western Blot (WB)
(Western Blot using MBS600727)
Mouse anti-Human, Mouse c-Abl Monoclonal Antibody | anti-ABL1 antibody
c-Abl (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; bcr/abl; ABL1
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
c-Abl; Monoclonal Antibody; c-Abl (ABL1; v-abl; Abelson Murine Leukemia Viral Oncogene Homolog 1; ABL; c-ABL; JTK7; p150; Proto-Oncogene Tyrosine-Protein Kanase ABL1; v-abl); Anti -c-Abl (ABL1; anti-ABL1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,g
Clone Number
2Q217
Specificity
Recognizes human c-Abl p120 and chimeric Bcr/Abl proteins.. Species crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also supplied without BSA and sodium azide. See C0013-02AX.
Sequence
SH2 domain. MW of Antigen: 120kD.
Applicable Applications for anti-ABL1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Dilution: Immunoprecipitation: 2ug/mg protein lysate (Use Protein G).
Western Blot: 1ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8 for 10-20 minutes followed by cooling at RT for 20 minutes.
Western Blot: 1ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8 for 10-20 minutes followed by cooling at RT for 20 minutes.
Immunogen
Recombinant Abl protein. Cellular Localization: Cytoplasmic and nuclear Epitope: SH2 domain. MW of Antigen: 120kD.
Positive Control
HeLa cells or placenta
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot using MBS600727)
Western Blot (WB)
(Western Blot using MBS600727)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human placenta stained with MBS600727 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human placenta stained with MBS600727 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.)
Related Product Information for anti-ABL1 antibody
Bcr-Abl hybrid gene product is a 210kD protein known to be assembled in K562 (human chronic myelogenous leukemia) cell line. c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both nucleus and cytoplasm of cells. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, c-abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as the result of the fusion of c-Abl sequences from chromosome 9 to Bcr gene on chromosome 22. The molecular consequence of translocation is generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1. The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains, which is conserved among the Abl family. Phosphorylation of Tyr245 is involved in activation of c-Abl kinase activity (5). Tyrosine 412 is located in the kinase activation loop of c-Abl molecules; phosphorylation of this residue is required for the kinase activity (6). Thr735 is in a conserved 14-3-3 protein binding motif, which can be phosphorylated upon stress stimulation or TPA treatment (Wu, J. et al. unpublished data). Phosphorylation of Thr735 in c-Abl may play an important role in regulation of c-Abl localization as well as its function.
Product Categories/Family for anti-ABL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
13,150 Da
NCBI Official Full Name
c-abl
NCBI Official Synonym Full Names
c-abl oncogene 1, non-receptor tyrosine kinase
NCBI Official Symbol
ABL1
NCBI Official Synonym Symbols
ABL; JTK7; p150; c-ABL; v-abl; bcr/abl; ABL1
NCBI Protein Information
tyrosine-protein kinase ABL1; proto-oncogene c-Abl; bcr/c-abl oncogene protein; c-abl oncogene 1, receptor tyrosine kinase; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolog 1
UniProt Protein Name
C-abl
Protein Family
UniProt Gene Name
ABL1
UniProt Entry Name
Q13915_HUMAN
NCBI Description
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq]
Uniprot Description
Sequence similarities: Contains 1 SH3 domain. RuleBase RU003338
Research Articles on ABL1
1. Among 55 imatinib-resistant CML patients treated with dastinib or nilotinib, baseline mutations in the kinase domain disappeared in 8 patients & new mutations appeared in 9. T315I was the most frequently detected mutation.
2. Observational study of genetic testing. (HuGE Navigator)
3. Overexpression of c-ABL proto-oncogene is associated with enhanced resistance to tamoxifen in breast cancer.
4. Mounting evidence summarized in this review suggests that tyrosine kinase c-Abl and Fyn are critical in the neurodegenerative process which occurs in tauopathies.
5. Review of ABL1 fusion genes identified in T-cell acute lymphoblastic leukemia that provide proliferation and survival advantage to lymphoblasts.
6. Study suggests important roles of BCR-ABL gene expression and its native chromosomal locus for acquisition of BCR-ABL mutations and provides a new tool for further studying resistance mechanisms.
7. Endogenous c-Abl mediates endothelial apoptosis induced by inhibition of integrins alphavbeta3/alphavbeta5 or by LatB-induced disruption of F-actin.
8. The results show that Bcr-Abl regulates the actin cytoskeleton and non-apoptotic membrane blebbing via a GADS/Slp-76/Nck1 adaptor protein pathway.
9. the additive effects of imatinib and Everolimus on BCR-ABL-expressing cells are at least partly conditional upon the enhanced nuclear accumulation of p145 c-ABL
10. Studiies indicate that KD mutations conferring high-level imatinib resistance are present in patients with de novo CML and in some of them lead to disease progression.
2. Observational study of genetic testing. (HuGE Navigator)
3. Overexpression of c-ABL proto-oncogene is associated with enhanced resistance to tamoxifen in breast cancer.
4. Mounting evidence summarized in this review suggests that tyrosine kinase c-Abl and Fyn are critical in the neurodegenerative process which occurs in tauopathies.
5. Review of ABL1 fusion genes identified in T-cell acute lymphoblastic leukemia that provide proliferation and survival advantage to lymphoblasts.
6. Study suggests important roles of BCR-ABL gene expression and its native chromosomal locus for acquisition of BCR-ABL mutations and provides a new tool for further studying resistance mechanisms.
7. Endogenous c-Abl mediates endothelial apoptosis induced by inhibition of integrins alphavbeta3/alphavbeta5 or by LatB-induced disruption of F-actin.
8. The results show that Bcr-Abl regulates the actin cytoskeleton and non-apoptotic membrane blebbing via a GADS/Slp-76/Nck1 adaptor protein pathway.
9. the additive effects of imatinib and Everolimus on BCR-ABL-expressing cells are at least partly conditional upon the enhanced nuclear accumulation of p145 c-ABL
10. Studiies indicate that KD mutations conferring high-level imatinib resistance are present in patients with de novo CML and in some of them lead to disease progression.
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Product Notes
The ABL1 abl1 (Catalog #AAA600727) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The c-Abl (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's c-Abl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Dilution: Immunoprecipitation: 2ug/mg protein lysate (Use Protein G). Western Blot: 1ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8 for 10-20 minutes followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the ABL1 abl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SH2 domain. MW of Antigen: 120kD. It is sometimes possible for the material contained within the vial of "c-Abl, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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