Testing Data
(Stain|Blot)
Mouse anti-Human, Rat E2F4 Transcription Factor Monoclonal Antibody | anti-E2F4 antibody
E2F4 Transcription Factor (BSA & Azide Free)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Immunofluorescence
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Testing Data
(Stain|Blot)
Testing Data
(Stain|Blot)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human tonsil stained with E2F-4 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human tonsil stained with E2F-4 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq]
Uniprot Description
E2F4: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Belongs to the E2F/DP family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: nucleoplasm; transcription factor complex
Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor binding; transcription factor activity
Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription initiation from RNA polymerase II promoter; organ morphogenesis; transcription, DNA-dependent; blood circulation; transforming growth factor beta receptor signaling pathway; epithelial cell development; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; cell volume homeostasis; regulation of cell size; regulation of cell proliferation
Research Articles on E2F4
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Product Notes
The E2F4 e2f4 (Catalog #AAA603812) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The E2F4 Transcription Factor (BSA & Azide Free) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's E2F4 Transcription Factor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Dilution: Gel Supershift Assay (Use Ab at 1mg/ml) Immunofluorescence Western Blotting (Ab 1-2ug/ml for 2hrs at RT) Immunohistochemistry (Formalin/paraffin) (Use Ab at 2-4ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Researchers should empirically determine the suitability of the E2F4 e2f4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Not determined. It is sometimes possible for the material contained within the vial of "E2F4 Transcription Factor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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