Western Blot (WB)
(Stain|Western Blot)
Mouse anti-Human Caldesmon, LMW Monoclonal Antibody
Caldesmon, LMW
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Caldesmon; LMW; Monoclonal Antibody; Anti -Caldesmon; anti-Caldesmon; LMW antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F41
Specificity
Recognizes Caldesmon, LMW. Species Crossreactivity: human.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C1032-40X
Sequence
Not determined
Applicable Applications for anti-Caldesmon, LMW antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Dilution: Immunofluorescence
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Immunogen
Recombinant caldesmon
Cellular Localization
Cytoplasmic
Positive Control
Rh30 cells. Uterus
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Stain|Western Blot)
Western Blot (WB)
(Stain|Western Blot)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human uterus stained with Caldesmon Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscles.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human uterus stained with Caldesmon Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscles.)
Related Product Information for anti-Caldesmon, LMW antibody
Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their eletrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kD) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kD) is found in nonmuscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.
Product Categories/Family for anti-Caldesmon, LMW antibody
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Product Notes
The Caldesmon, LMW (Catalog #AAA600194) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Caldesmon, LMW reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caldesmon, LMW can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Dilution: Immunofluorescence Western Blotting (Ab 1-2ug/ml for 2hrs at RT) Immunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Researchers should empirically determine the suitability of the Caldesmon, LMW for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Not determined. It is sometimes possible for the material contained within the vial of "Caldesmon, LMW, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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