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Testing Data
Mouse anti-Human, Rat XRCC1 Monoclonal Antibody | anti-XRCC1 antibody
XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free)
Gene Names
XRCC1; RCC
Reactivity
Human, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
XRCC1; Monoclonal Antibody; XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free); Anti -XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free); anti-XRCC1 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3F399
Specificity
Recognizes XRCC1 (X-Ray Repair Cross Complementing Protein1). Species Crossreactivity: human and rat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See X9501-01
Sequence
Not determined
Applicable Applications for anti-XRCC1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Dilution: Immunoprecipitation (Native verified) (Use Protein A) (Ab 2ug/mg protein lysate)
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (1-2ug/ml for 30 min at RT; is better) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Western Blotting (Ab 1-2ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin) (1-2ug/ml for 30 min at RT; is better) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Immunogen
Recombinant human XRCC1 protein
Cellular Localization
Nuclear
Positive Control
Ls174T, HT29, or HeLa cells. Normal testis.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
Testing Data
Testing Data
Testing Data
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded rat testis stained with XRCC1 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded rat testis stained with XRCC1 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.)
Testing Data
Testing Data
Related Product Information for anti-XRCC1 antibody
X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half.. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.
Product Categories/Family for anti-XRCC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
85kD
NCBI Official Full Name
XRCC1
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 1
NCBI Official Symbol
XRCC1
NCBI Official Synonym Symbols
RCC
NCBI Protein Information
DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster
UniProt Protein Name
DNA repair protein XRCC1
Protein Family
UniProt Gene Name
XRCC1
UniProt Entry Name
XRCC1_HUMAN
NCBI Description
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq]
Uniprot Description
XRCC1: Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.
Protein type: DNA repair, damage
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; enzyme binding; damaged DNA binding
Biological Process: single strand break repair; base-excision repair; DNA repair
Research Articles on XRCC1
Similar Products
Product Notes
The XRCC1 xrcc1 (Catalog #AAA600757) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's XRCC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Dilution: Immunoprecipitation (Native verified) (Use Protein A) (Ab 2ug/mg protein lysate) Western Blotting (Ab 1-2ug/ml for 2hrs at RT) Immunohistochemistry (Formalin/paraffin) (1-2ug/ml for 30 min at RT; is better) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Researchers should empirically determine the suitability of the XRCC1 xrcc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Not determined. It is sometimes possible for the material contained within the vial of "XRCC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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