Mouse anti-Human, Rat E2F4 Transcription Factor Monoclonal Antibody | anti-E2F4 antibody

E2F4 Transcription Factor

Cat. Num. AAA600890

• Gene Names: E2F4; E2F-4
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: E2F4 Transcription Factor; Monoclonal Antibody; Anti -E2F4 Transcription Factor; anti-E2F4 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3F377
• Specificity: Recognizes Transcription Factor E2F4. Species Crossreactivity: Human and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E0014-02X.

• Applicable Applications for anti-E2F4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Gel Supershift, Immunofluorescence, Western Blot and Immunohistochemistry.; Dilution: Gel Supershift Assay: 1mg/ml; Western Blot: 1-2ug/ml for 2hrs at RT; Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 min at RT; Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunogen: Recombinant human E2F-4 protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: ~56kD
• Cellular Localization: Nuclear and cytoplasmic
• Positive Control: Raji cells and Tonsil.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Stain|Blot)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human tonsil stained with E2F-4 Ab using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.)

Related Product Information for anti-E2F4 antibody

E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides Differential phosphorylation contributes to the microheterogeniety of E2F-4 (total aa411-416, varies due to a trinucleotide repeat).

Product Categories/Family for anti-E2F4 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7637752
• NCBI GeneID: 1874
• UniProt Accession #: Q16254; Q12991; Q15328; A6NGR8; B5BU56
• Molecular Weight: ~56kD
• NCBI Official Full Name: E2F transcription factor 4
• NCBI Official Synonym Full Names: E2F transcription factor 4, p107/p130-binding
• NCBI Official Symbol: E2F4
• NCBI Official Synonym Symbols: E2F-4
• NCBI Protein Information: transcription factor E2F4; p107/p130-binding protein
• UniProt Protein Name: Transcription factor E2F4
• Protein Family: Transcription factor
• UniProt Gene Name: E2F4
• UniProt Entry Name: E2F4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq]

Uniprot Description

E2F4: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Belongs to the E2F/DP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity; transcription factor binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription initiation from RNA polymerase II promoter; organ morphogenesis; transcription, DNA-dependent; blood circulation; transforming growth factor beta receptor signaling pathway; epithelial cell development; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; cell volume homeostasis; regulation of cell size; regulation of cell proliferation