Mouse anti-Human VCAM-1 Monoclonal Antibody | anti-VCAM-1 antibody

VCAM-1, Human, mAb 1G11B1, biotinylated

Cat. Num. AAA584876

• Gene Names: Vcam1; VCAM1B
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence
• Synonyms: VCAM-1; Monoclonal Antibody; Human; mAb 1G11B1; biotinylated; CD106; INCAM-100; L1CAM; MGC99561; DKFZp779G2333;; VCAM-1 Human mAb 1G11B1 Biotin 50ug; anti-VCAM-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Form/Format: 0.5ml (100ug/ml) 0.2um filtered biotinylated antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
• Sequence Length: 739

• Applicable Applications for anti-VCAM-1 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen, Functional Studies (FS), Immunoassay (IA), Immunofluorescence (IF)
• Application Notes: The typical starting working dilution is 1:50.; IHC-F: Cryostatic sections were fixed in acetone, and incubated with mAb at 10ug/ml in PBS/0.1% tween20; FC: 1ug mAb per 1x105 cells; FS: Stimulated EC were preincubated for 30' at 37 degree C with mAb and than used in adhesion assay with T-cells; IP: precleared EC lysates were immunoprecipitated with mAB and analyzed by 7.5% SDS-PAGE under reducing conditions
• Immunogen: Full length protein
• Conjugation: Biotin
• Positive Control: Activated endothelial cells
• Preparation and Storage: Product should be stored at 4 degree C.; Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-VCAM-1 antibody

The monoclonal antibody 1G11B1 recognizes vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 is a member of the immunoglobulin superfamily of adhesion molecules, which includes ICAMs, PECAM-s and MADCAM, and is involved in leukocyte-endothelial cell interactions. The immunoglobulin superfamily is a type I transmembrane protein characterized by extracellular immunoglobin domains, a transmembrane region and a cytoplasmic tail. They are essential for the development of the embryo and for immune and inflammatory responses. These transmembrane glycoproteins mediate cell interaction with, and adhesion to, other cells and the extracellular matrix. VCAM-1 contains six immunoglobulin domains of the H-type and interacts with VLA-4 expressed on leukocytes. Multiple adhesion molecules play a role in leukocyte recruitment. The process of migration of a leukocyte through the vascular endothelium consists of the following steps: leukocyte-endothelium interaction (first tethering and rolling and than adhesion) and transendothelial migration. VCAM-1 is almost not expressed under physiological conditions. However, under appropriate pro-inflammatory conditions where the endothelium is exposed to inflammatory cytokines such as tumour necrosis factor-alpha or IL-1b and becomes activated, VCAM-1 gene expression is rapid elevated by the vascular endothelium. There is also a soluble form of VCAM-1 which is angiogenic and chemotactic for endothelial cells. sVCAM-1 is up-regulated in several disease states (eg, myocardial infarction, type 2 diabetes mellitus, primary antiphospholipid syndrome, and rheumatoid arthritis).

Product Categories/Family for anti-VCAM-1 antibody

Cardiology and metabolism, Tumor immunology

References

1. Thornhill, M et al; Tumor necrosis factor combines with IL-4 or IFN-gamma to selectively enhance endothelial cells adhesiveness for T cells. The contribution of vascular cell adhesion molecule-1-dependent and -independent binding mechanisms. J Immunology 1991, 146: 592
2. Van der Vieren, M. et al; The Leukocyte Integrin alpha4beta2- binds VCAM-1: Evidence for a binding interface between I domain and VCAM-1. J Immunol 1999, 163:1984
3. Dienst, A et al; Specific occlusion of murine andHuman tumor vasculature by VCAM-1-Targeted recombinant fusion protein. J Natl Cancer Inst 2005, 97 :733
4. Wijbrandt, C et al; The clinical response to infliximab in rheumatoid arthritis is in part dependent on pretreatment tumour necrosis factor ; expression in the synovium. Ann Rheum Dis 2008, 67:1139
5. Roussel, L et al; IL-17 Promotes p38 MAPK-dependent endothelial activation enhancing- neutrophil recruitment to sites of inflammation. J immunol. 2010, 184:4531

NCBI and Uniprot Product Information

• NCBI GI #: 6981700
• NCBI GeneID: 25361
• NCBI GenBank Nucleotide #: NP_037021.1
• UniProt Accession #: P29534
• NCBI Official Full Name: vascular cell adhesion protein 1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: Vcam1
• NCBI Official Synonym Symbols: VCAM1B
• NCBI Protein Information: vascular cell adhesion protein 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• UniProt Gene Name: Vcam1
• UniProt Synonym Gene Names: Vcam-1; V-CAM 1; VCAM-1

NCBI Description

binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]

Uniprot Description

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.