Rabbit anti-Chicken Ovomucoid Polyclonal Antibody | anti-SPIK7 antibody

Rabbit anti Ovomucoid Polyclonal Antibody

Cat. Num. AAA460400

• Gene Names: SPIK7; OM; OVM; SPINK7
• Reactivity: Chicken
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Ovomucoid; Polyclonal Antibody; Rabbit anti Ovomucoid Polyclonal Antibody; OVM; OM; anti-SPIK7 antibody

• Host: Rabbit
• Reactivity: Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~28 kDa of chicken egg white Ovomucoid protein.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 210

• Applicable Applications for anti-SPIK7 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to the C-terminus of chicken egg white Ovomucoid protein.
• Positive Control: Ovomucoid
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot (WB)

(The highly purified Ovomucoid protein from chicken egg white was immunoblotted by Rabbit anti Ovomucoid antibody (Cat#MBS460400) at 1:500. An immunoreactive band is observed at ~28 kDa.)

Related Product Information for anti-SPIK7 antibody

Ovomucoid is isolated from chicken egg white. The precursor is a 210 aa secreted protein which consists of three homologous, tandem Kazal family inhibitory domains each homologous to pancreatic secretory trypsin inhibitor (Kazal) and each with an actual or putative reactive site for inhibition of serine proteinases. The major reactive site for bovine beta-trypsin is the Arg89-Ala peptide bond in the second domain. It causes an allergic reaction in human. Allergy to chicken egg or proteins is one of the most frequent causes of food hypersensitivity in infants and young children. Both IgG and IgA class antibodies may be detected. Ovalbumin intolerance has been implicated in a number of conditions affecting children. In particular, children with cystic fibrosis show elevated anti-Ovalbumin antibodies. Ovalbumin antibodies have also been noted in some forms of kidney disease. A relationship between food allergy and infantile autism has also been observed. Children with insulin-dependent diabetes mellitus show an enhanced immune response to both beta- actoglobulin and ovalbumin, a phenomenon that may be related to the development of the disease. Conditions related to ovalbumin intolerance usually resolve once egg and egg based foods have been withdrawn from the patient's diet. Intolerance to egg proteins could be due not only to the ovalbumin protein found in egg white but also to other major proteins present in the yolk. The major proteins of chicken eggs are: Ovalbumin (45 kda, 54%), Conalbumin (13%, 80 kda), Ovomucoid (11%, 28 kda), Lysozyme (3.5%, 14 kda), and other proteins.

References

Tsukasa Matsuda, Kenji Watanabe, and Ryo Nakamura. Ovomucoid and ovoinhibitor isolated from chicken egg white are immunologically cross-reactive.

NCBI and Uniprot Product Information

• NCBI GI #: 819231687
• NCBI GeneID: 416236
• NCBI Accession #: NP_001295423.1
• NCBI GenBank Nucleotide #: NM_001308494.1
• UniProt Accession #: P01005
• Molecular Weight: ~28 kDa
• NCBI Official Full Name: ovomucoid isoform 1
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kazal type 7 (putative)
• NCBI Official Symbol: SPIK7
• NCBI Official Synonym Symbols: OM; OVM; SPINK7
• NCBI Protein Information: ovomucoid
• UniProt Protein Name: Ovomucoid
• Protein Family: Ovomucoid

Uniprot Description

Serine protease inhibitor. Inhibits trypsin.