Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Vascular cell adhesion molecule 1 (VCAM-1) Standard Curve.)
Rat Vascular Cell Adhesion Molecule 1 (VCAM-1) ELISA Kit | VCAM-1 elisa kit
Rat Vascular Cell Adhesion Molecule 1 (VCAM-1) ELISA Kit
Gene Names
Vcam1; VCAM1B
Reactivity
Rat
Synonyms
Vascular Cell Adhesion Molecule 1 (VCAM-1); Rat Vascular Cell Adhesion Molecule 1 (VCAM-1) ELISA Kit; VCAM1; CD106; DKFZp779G2333; INCAM-100; MGC99561; CD106 antigen; VCAM-1 elisa kit
Reactivity
Rat
Specificity
Rat Vascular cell adhesion molecule 1 (VCAM-1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat VCAM1. No significant cross-reactivity or interference between Rat VCAM1 and analogues was observed.
Sequence Length
739
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Vascular cell adhesion molecule 1 (VCAM-1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Vascular cell adhesion molecule 1 (VCAM-1) Standard Curve.)
Related Product Information for VCAM-1 elisa kit
Description: This Rat Vascular cell adhesion molecule 1 (VCAM-1) ELISA Kit employs a two-site sandwich ELISA to quantitate VCAM1 in samples. An antibody specific for VCAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha4beta7. The rat and mouse SVCAM-1 proteins share approximately 76% amino acid similarity. VCAM-1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis.
Background: VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha4beta7. The rat and mouse SVCAM-1 proteins share approximately 76% amino acid similarity. VCAM-1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
Vcam1
NCBI Official Synonym Symbols
VCAM1B
NCBI Protein Information
vascular cell adhesion protein 1
UniProt Protein Name
Vascular cell adhesion protein 1
UniProt Gene Name
Vcam1
UniProt Synonym Gene Names
Vcam-1; V-CAM 1; VCAM-1
NCBI Description
binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]
Uniprot Description
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Research Articles on VCAM-1
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Product Notes
The Rat VCAM-1 vcam1 (Catalog #AAA9711614) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711614 ELISA Kit recognizes Rat VCAM-1. It is sometimes possible for the material contained within the vial of "Vascular Cell Adhesion Molecule 1 (VCAM-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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