Rat vascular cell adhesion protein 1 (VCAM-1/CD106) ELISA Kit | VCAM-1/CD106 elisa kit

Rat vascular cell adhesion protein 1, VCAM-1/CD106 ELISA Kit

Cat. Num. AAA703064

• Gene Names: Vcam1; VCAM1B
• Reactivity: Rat
• Synonyms: vascular cell adhesion protein 1 (VCAM-1/CD106); Rat vascular cell adhesion protein 1; VCAM-1/CD106 ELISA Kit; Rat vascular cell adhesion protein 1 (VCAM-1/CD106) ELISA kit; CD106; DKFZp779G2333; INCAM-100; MGC99561; CD106 antigen; VCAM-1/CD106 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat VCAM-1. No significant cross-reactivity or interference between rat VCAM-1 and analogues was observed.
• Sequence Length: 739

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 15.6 ng/ml -1000 ng/mL
• Sensitivity: <3.9 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VCAM-1/CD106 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VCAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCAM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6981700
• NCBI GeneID: 25361
• NCBI Accession #: NP_037021.1
• NCBI GenBank Nucleotide #: NM_012889.1
• UniProt Accession #: P29534; Q5FVS3; Q63669
• Molecular Weight: 81,246 Da
• NCBI Official Full Name: vascular cell adhesion protein 1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: Vcam1
• NCBI Official Synonym Symbols: VCAM1B
• NCBI Protein Information: vascular cell adhesion protein 1; VCAM-1; V-CAM 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• UniProt Gene Name: Vcam1
• UniProt Synonym Gene Names: Vcam-1; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_RAT

NCBI Description

binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]

Uniprot Description

Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.