Mouse VCAM-1 ELISA Kit | VCAM-1 elisa kit

VCAM-1 Mouse ELISA Kit

Cat. Num. AAA355350

• Gene Names: Vcam1; VCAM1B
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: VCAM-1; VCAM-1 Mouse ELISA Kit; Mouse VCAM-1; VCAM-1 elisa kit

• Reactivity: Mouse

• Applicable Applications for VCAM-1 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of VCAM-1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 156 pg/ml-10,000 pg/ml
• Sensitivity: < 5pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VCAM-1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-VCAM-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-VCAM-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VCAM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VCAM-1 can be calculated.

Background: Vascular cell adhesion protein 1 (VCAM-1), also known as cluster of differentiation 106 (CD106) is a protein that in humans is encoded by the VCAM1 gene. This gene contains 9 exons spanning about 25 kb, and It is expressed on both large and small blood vessels only after the endothelial cells are stimulated by cytokines. The VCAM-1 is a cell surface sialoglycoprotein, a type I membrane protein that is a member of the Ig superfamily. It mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development of atherosclerosis and rheumatoid arthritis.

NCBI and Uniprot Product Information

• NCBI GI #: 6981700
• NCBI GeneID: 25361
• NCBI Accession #: NP_037021.1
• NCBI GenBank Nucleotide #: NM_012889.1
• UniProt Accession #: P29534; Q5FVS3; Q63669
• Molecular Weight: 81,246 Da
• NCBI Official Full Name: vascular cell adhesion protein 1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: Vcam1
• NCBI Official Synonym Symbols: VCAM1B
• NCBI Protein Information: vascular cell adhesion protein 1; VCAM-1; V-CAM 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• UniProt Gene Name: Vcam1
• UniProt Synonym Gene Names: Vcam-1; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_RAT

NCBI Description

binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]

Uniprot Description

Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Subunit structure: Binds to ECMV-D capsid proteins and acts as a receptor for this virus

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Sequence similarities: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.