Typical Testing Data/Standard Curve (for reference only)
(Mouse VCAM-1 PicoKine ELISA Kit standard curve )
Mouse VCAM-1 ELISA Kit | VCAM1 elisa kit
Mouse VCAM-1 PicoKine ELISA Kit
Gene Names
Vcam1; CD106; Vcam-1
Reactivity
Mouse
Synonyms
VCAM-1; Mouse VCAM-1 PicoKine ELISA Kit; Vascular cell adhesion protein 1; vascular cell adhesion molecule 1; CD106; CD106 antigen; DKFZp779G2333; INCAM 100; INCAM-100; INCAM100; L1CAM; MGC99561; V CAM 1; V-CAM 1; Vascular cell adhesion molecule 1; VCAM 1; VCAM1; VCAM1_HUMAN; VCAM1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse VCAM-1
Sequence Length
739
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 5pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse VCAM-1 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse VCAM-1 PicoKine ELISA Kit standard curve )
Related Product Information for VCAM1 elisa kit
Principle of the assay: mouse VCAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VCAM-1 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VCAM-1 amount of sample captured in plate.
Background: Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.
Background: Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
38,372 Da
NCBI Official Full Name
Vascular cell adhesion protein 1
NCBI Official Synonym Full Names
vascular cell adhesion molecule 1
NCBI Official Symbol
Vcam1
NCBI Official Synonym Symbols
CD106; Vcam-1
NCBI Protein Information
vascular cell adhesion protein 1; V-CAM 1
UniProt Protein Name
Vascular cell adhesion protein 1
Protein Family
UniProt Gene Name
Vcam1
UniProt Synonym Gene Names
Vcam-1; V-CAM 1; VCAM-1
UniProt Entry Name
VCAM1_MOUSE
Uniprot Description
VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; early endosome; integral to membrane; membrane; apical part of cell; plasma membrane; podosome; sarcolemma; external side of plasma membrane; filopodium
Molecular Function: amine oxidase activity; integrin binding; cell adhesion molecule binding
Biological Process: heterophilic cell adhesion; leukocyte adhesion; cell-cell adhesion; viral reproduction; heart development; cell-matrix adhesion; positive regulation of T cell proliferation; amine metabolic process; cell adhesion; leukocyte tethering or rolling
Research Articles on VCAM1
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Product Notes
The Mouse VCAM1 vcam1 (Catalog #AAA175928) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175928 ELISA Kit recognizes Mouse VCAM1. It is sometimes possible for the material contained within the vial of "VCAM-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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