Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells, untreated or treated with L7751 LY294002, insulin, or both using MBS6011344 (upper) or total NDRG1 antibody (lower).)
Rabbit N-myc Downstream Regulated Gene 1 Protein Monoclonal Antibody | anti-NDRG1 antibody
N-myc Downstream Regulated Gene 1 Protein, phosphorylated (Thr346) (NDRG1, Ndr1, Ndrl, Protein NDRG1, Protein Ndr1, CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, Tdd5)
Purified
Purified
Dilution: Immunofluorescence: 1:800
Flow Cytometry: 1:200
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X
TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:500
Immunocytochemistry: 1:800
Immunoprecipitation: 1:100
Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells, untreated or treated with L7751 LY294002, insulin, or both using MBS6011344 (upper) or total NDRG1 antibody (lower).)
Western Blot (WB)
(Western Blot analysis of extracts from C2C12 cells, untreated or treated with L7751 LY294002, insulin, or both using MBS6011344 (upper) or total NDRG1 antibody (lower).)
Western Blot (WB)
(Western Blot analysis of HeLa cells treated with hEGF (100ng/ml, 30 minutes), mock transfected or transfected with NDRG1 siRNA I or NDRG1 siRNA II, using MBS6011344 (upper) or 376992 beta-Tubulin Rabbit mAb (lower).)
Western Blot (WB)
(Western Blot analysis of HeLa cells treated with hEGF (100ng/ml, 30 minutes), mock transfected or transfected with NDRG1 siRNA I or NDRG1 siRNA II, using MBS6011344 (upper) or 376992 beta-Tubulin Rabbit mAb (lower).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of C2C12 cells, treated with L7751 LY294002(left) or insulin (right), using MBS6011344 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of C2C12 cells, treated with L7751 LY294002(left) or insulin (right), using MBS6011344 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis on Phospho-Akt (Ser473) IHC Controls [paraffin-embedded LNCaP cell pellets, control (left) or LY294002-treated (right)] using NDRG1 Antibody (top) or MBS6011344 (bottom).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis on Phospho-Akt (Ser473) IHC Controls [paraffin-embedded LNCaP cell pellets, control (left) or LY294002-treated (right)] using NDRG1 Antibody (top) or MBS6011344 (bottom).)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells, untreated (green) or treated with L7751 LY294002, wortmannin and U0126 (blue), using MBS6011344.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of Jurkat cells, untreated (green) or treated with L7751 LY294002, wortmannin and U0126 (blue), using MBS6011344.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Uniprot Description
NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D).
Protein type: Vesicle; Cell development/differentiation
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: microtubule cytoskeleton; centrosome; microtubule; cell-cell adherens junction; recycling endosome membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; cytosol
Molecular Function: protein binding; gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding
Biological Process: response to metal ion; DNA damage response, signal transduction by p53 class mediator; myelin maintenance in the peripheral nervous system; mast cell activation
Disease: Charcot-marie-tooth Disease, Type 4d
Research Articles on NDRG1
Similar Products
Product Notes
The NDRG1 ndrg1 (Catalog #AAA6011344) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The N-myc Downstream Regulated Gene 1 Protein, phosphorylated (Thr346) (NDRG1, Ndr1, Ndrl, Protein NDRG1, Protein Ndr1, CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, Tdd5) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's N-myc Downstream Regulated Gene 1 Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Dilution: Immunofluorescence: 1:800 Flow Cytometry: 1:200 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:500 Immunocytochemistry: 1:800 Immunoprecipitation: 1:100. Researchers should empirically determine the suitability of the NDRG1 ndrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-myc Downstream Regulated Gene 1 Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
