Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence: MBS602233 was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).)
Mouse RNA 5.8s, Ribosomal Monoclonal Antibody
RNA 5.8s, Ribosomal (Ribosomal RNA 5.8s, rRNA 5.8s)
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
RNA 5.8s; Ribosomal; Monoclonal Antibody; Ribosomal (Ribosomal RNA 5.8s; rRNA 5.8s); Anti -RNA 5.8s; anti-RNA 5.8s; Ribosomal antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
9C93
Specificity
This antibody is specific for ribosomal RNA. There is no crossreactivity with other RNAs. Species Crossreactivity: This antibody reacts with all eukaryotes.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RNA 5.8s, Ribosomal antibody
Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Recommended Dilutions: Immunofluorescence: 1:2500
Immunoprecipitation:10 ul/lysate
Immunocytochemistry: Frozen, paraffin
Optimal dilutions to be determined by the researcher.
Immunoprecipitation:10 ul/lysate
Immunocytochemistry: Frozen, paraffin
Optimal dilutions to be determined by the researcher.
Immunogen
The whole 5.8S ribosomal RNA.
Cellular Localization
cytoplasmic, nuclear
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifugethe original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence: MBS602233 was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).)
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence: MBS602233 was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).)
Immunofluorescence (IF)
(Immunocytochemistry/Immunofluorescence: Staining of rRNA in vero cells using MBS602233.)
Immunofluorescence (IF)
(Immunocytochemistry/Immunofluorescence: Staining of rRNA in vero cells using MBS602233.)
Related Product Information for anti-RNA 5.8s, Ribosomal antibody
The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.
This antibody is useful for recognizing RNA epitopes on both the large and small subunit.
This antibody is useful for recognizing RNA epitopes on both the large and small subunit.
Product Categories/Family for anti-RNA 5.8s, Ribosomal antibody
Similar Products
Product Notes
The RNA 5.8s, Ribosomal (Catalog #AAA602233) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RNA 5.8s, Ribosomal can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF). Recommended Dilutions: Immunofluorescence: 1:2500 Immunoprecipitation:10 ul/lysate Immunocytochemistry: Frozen, paraffin Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the RNA 5.8s, Ribosomal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA 5.8s, Ribosomal, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
