Human PLA2G4A ELISA Kit | PLA2G4A elisa kit

Human PLA2G4A PicoKine ELISA Kit

Cat. Num. AAA1752670

• Gene Names: PLA2G4A; GURDP; cPLA2; PLA2G4; cPLA2-alpha
• Reactivity: Human
• Synonyms: PLA2G4A; Human PLA2G4A PicoKine ELISA Kit; Human PLA2G4A; phospholipase A2 group IVA; Cytosolic phospholipase A2; Cpla2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase; CPLA2; PLA2G4; PLA2G4A elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human PLA2G4A
• Sequence Length: 749

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLA2G4A elisa kit

Principle of the Assay: The Picokine™ Human PLA2G4A Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PLA2G4A with a 96-well strip plate that is pre-coated with antibody specific for PLA2G4A. The detection antibody is a biotinylated antibody specific for PLA2G4A. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human PLA2G4A with immunogen: Expression system for standard: NSO, Immunogen sequence: M1-A749. The kit is analytically validated with ready to use reagents. To measure Human PLA2G4A, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human PLA2G4A in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human PLA2G4A in the sample.!!Background/Introduction: Cytosolic phospholipase A2 is an enzyme that in humans is encoded by the PLA2G4A gene. It is mapped to 1q31.1. This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca2+ levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.

NCBI and Uniprot Product Information

• NCBI GI #: 23943920
• NCBI GeneID: 5321
• NCBI Accession #: NP_077734.1
• NCBI GenBank Nucleotide #: NP_077734.1
• UniProt Accession #: P47712
• NCBI Official Full Name: cytosolic phospholipase A2 isoform 1
• NCBI Official Synonym Full Names: phospholipase A2 group IVA
• NCBI Official Symbol: PLA2G4A
• NCBI Official Synonym Symbols: GURDP; cPLA2; PLA2G4; cPLA2-alpha
• NCBI Protein Information: cytosolic phospholipase A2
• UniProt Protein Name: Cytosolic phospholipase A2
• Protein Family: Cytosolic phospholipase
• UniProt Gene Name: PLA2G4A
• UniProt Synonym Gene Names: CPLA2; PLA2G4; cPLA2
• UniProt Entry Name: PA24A_HUMAN

NCBI Description

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: Lipid Metabolism - ether lipid; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; EC 3.1.1.5; Phospholipase; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasm; cytoplasmic membrane-bound vesicle; mitochondrial inner membrane; cytosol

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity

Biological Process: icosanoid biosynthetic process; platelet activating factor biosynthetic process; platelet activation; phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; icosanoid metabolic process; phosphatidic acid biosynthetic process; arachidonic acid metabolic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation