Rabbit anti-Human, Rat PHD3 Polyclonal Antibody

PHD3 (Prolyl Hydroxylase Domain Containing Protein 3, HIF-Prolyl Hydroxylase 3, EGLN3)

Cat. Num. AAA612077

• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PHD3; Polyclonal Antibody; PHD3 (Prolyl Hydroxylase Domain Containing Protein 3; HIF-Prolyl Hydroxylase 3; EGLN3); Anti -PHD3 (Prolyl Hydroxylase Domain Containing Protein 3; anti-PHD3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HIF prolyl hydroxylase 3 at ~27kD, representing HIF prolyl hydroxylase 3. This antibody has been tested in 293 cell lysates.. Species Crossreactivity: rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.

• Applicable Applications for anti-PHD3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:2500.; Optimal dilution to be determined by the researcher.
• Immunogen: A synthetic peptide corresponding to a region between residue 50-100 of human prolyl hydroxylase domain-containing protein 3. [LocusLink ID 112399]
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of HeLa lysates using MBS612077.)

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence: MBS612077 was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).)

Western Blot (WB)

(Detection of Human PHD3 by Western Blot application in lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. MBS612077 was used at a dilution of 1:1000 and blots were developed using chemiluminescence.)

Western Blot (WB)

(Detection of PHD3 by Western Blot application in lysate from Rat cells before or 9 hours after inducing hypoxia. MBS612077 was used at a dilution of 1:5000 and blots were developed using chemiluminescence.)

Related Product Information for anti-PHD3 antibody

HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-1, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(564) and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may also play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissues, promoting cell death through a caspase-dependent mechanism.

Product Categories/Family for anti-PHD3 antibody

Antibodies; Abs to Hypoxia

NCBI and Uniprot Product Information

• NCBI GI #: 116563477
• NCBI Official Full Name: PHD3