Mouse anti-Mouse NFkB Activating Kinase Monoclonal Antibody | anti-NDFIP1 antibody

NFkB Activating Kinase (Nuclear Factor kappa B, TANK-Binding Kinase 1, NAK, TBK1)

Cat. Num. AAA604330

• Gene Names: NDFIP1; N4WBP5
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: NFkB Activating Kinase; Monoclonal Antibody; NFkB Activating Kinase (Nuclear Factor kappa B; TANK-Binding Kinase 1; NAK; TBK1); Anti -NFkB Activating Kinase (Nuclear Factor kappa B; anti-NDFIP1 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4H209
• Specificity: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recently, a new IKK family member, TBK1 (TANK-binding kinase) has been identified (8). TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-NDFIP1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml; Western Blot: 1-2ug/ml
• Immunogen: Human NFkB Activating Kinase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: TBK1 MBS604330 - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed withMBS604330 at 2ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry-Paraffin: N2301-40B] - Human testis (Formalin-Fixed Paraffin-Embedded (FFPE).)

Immunohistochemistry (IHC)

(Immunohistochemistry: MBS604330 - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A dilution of 1:10 was used. Image objective 40X.)

Related Product Information for anti-NDFIP1 antibody

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recently, a new IKK family member, TBK1 (TANK-binding kinase) has been identified (8). TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.

Product Categories/Family for anti-NDFIP1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 74733098
• NCBI GeneID: 80762
• UniProt Accession #: Q9BT67; Q658T8; Q8N2E3; Q8N2F9; B2RDB8; D3DQF0
• Molecular Weight: 24,899 Da
• NCBI Official Full Name: NEDD4 family-interacting protein 1
• NCBI Official Synonym Full Names: Nedd4 family interacting protein 1
• NCBI Official Symbol: NDFIP1
• NCBI Official Synonym Symbols: N4WBP5
• NCBI Protein Information: NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; breast cancer-associated protein SGA-1M; putative NFKB and MAPK-activating protein; putative NF-kappa-B-activating protein 164
• UniProt Protein Name: NEDD4 family-interacting protein 1
• Protein Family: NEDD4 family-interacting protein
• UniProt Gene Name: NDFIP1
• UniProt Synonym Gene Names: N4WBP5
• UniProt Entry Name: NFIP1_HUMAN

NCBI Description

The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

NDFIP1: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: Golgi membrane; perinuclear region of cytoplasm; extracellular region; integral to membrane; endosome membrane; cell cortex

Molecular Function: protein binding; signal transducer activity; WW domain binding

Biological Process: negative regulation of interleukin-4 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; cellular iron ion homeostasis; regulation of lymphocyte differentiation; regulation of myeloid leukocyte differentiation; signal transduction; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; negative regulation of T-helper 2 type immune response; positive regulation of protein ubiquitination; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein transport; negative regulation of transporter activity; regulation of isotype switching to IgG isotypes