Goat anti-Human Ribosomal Protein L8 Polyclonal Antibody | anti-RPL8 antibody

Ribosomal Protein L8 (60S Ribosomal Protein L8, L8, RPL8)

Cat. Num. AAA629812

• Gene Names: RPL8; L8
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: Ribosomal Protein L8; Polyclonal Antibody; Ribosomal Protein L8 (60S Ribosomal Protein L8; L8; RPL8); Anti -Ribosomal Protein L8 (60S Ribosomal Protein L8; anti-RPL8 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPL8. Species sequence homology: mouse, rat, canine.
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-RPL8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry; Dilution: Peptide ELISA Titer: 1:128,000; Western Blot: 0.01-0.03ug/ml ~28-30kD band observed in lysates of cell line HepG2 (calculated MW of 28.0kD according to NP_000964.1 and NP_150644.1); Immunohistochemistry: 2-4ug/ml In paraffin embedded Human Cerebral Cortex shows cytoplasm staining, including axons, of stallate and pyramidal cells.
• Immunogen: Synthetic peptide corresponding to KAGRAYHKYKAKRNC, from the internal region of human RPL8 (NP_000964.1; NP_150644.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS629812 (0.01µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

Immunohistochemistry (IHC)

(MBS629812 (2.5µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Related Product Information for anti-RPL8 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPL8 antibody

Antibodies; Abs to Ribosomal Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 433899
• NCBI GeneID: 6132
• UniProt Accession #: P62917; P25120; Q567Q7; Q969V7; Q9BWQ9; A8K094; D3DWN2
• Molecular Weight: 28,025 Da
• NCBI Official Full Name: ribosomal protein L8
• NCBI Official Synonym Full Names: ribosomal protein L8
• NCBI Official Symbol: RPL8
• NCBI Official Synonym Symbols: L8
• NCBI Protein Information: 60S ribosomal protein L8; OTTHUMP00000229065; OTTHUMP00000229066; OTTHUMP00000229067; OTTHUMP00000229068; OTTHUMP00000229069; OTTHUMP00000229071; OTTHUMP00000229072
• UniProt Protein Name: 60S ribosomal protein L8
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL8
• UniProt Entry Name: RL8_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Uniprot Description

RPL8: a ribosomal protein of the L2P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Associates with 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface.

Protein type: Ribosomal; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; cytosol

Molecular Function: rRNA binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; viral infectious cycle; translational termination