Western blot (WB)
(Whole cell extracts (15ug protein) from untreated (-) or nocodazole-treated (+, 300ng/ml overnight) HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1 ug/mL (1:500 dilution) of MBS6011004, overnight at 4°C. Proteins were visualized by chemiluminescence detection using IgG (HRP) goat anti-mouse antibody (1:3000). Direct-Blot™ anti-Beta-Actin was used as a loading control at a 1:25,000. Lane M: 'Molecular Weight Marker.)
Rat anti-Human Histone H3 Monoclonal Antibody
Histone H3, phosphorylated (Ser28) (Histone H3.1, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, H
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Western Blot: 0.5-1ug/ml.
Optimal dilutions to be determined by the researcher.
Western blot (WB)
(Whole cell extracts (15ug protein) from untreated (-) or nocodazole-treated (+, 300ng/ml overnight) HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1 ug/mL (1:500 dilution) of MBS6011004, overnight at 4°C. Proteins were visualized by chemiluminescence detection using IgG (HRP) goat anti-mouse antibody (1:3000). Direct-Blot™ anti-Beta-Actin was used as a loading control at a 1:25,000. Lane M: 'Molecular Weight Marker.)
Western blot (WB)
(Whole cell extracts (15ug protein) from untreated (-) or nocodazole-treated (+, 300ng/ml overnight) HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1 ug/mL (1:500 dilution) of MBS6011004, overnight at 4°C. Proteins were visualized by chemiluminescence detection using IgG (HRP) goat anti-mouse antibody (1:3000). Direct-Blot™ anti-Beta-Actin was used as a loading control at a 1:25,000. Lane M: 'Molecular Weight Marker.)
Immunofluorescence (IF)
(HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with MBS6011004 (1:2500) (0.2ug/ml) (lower) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG incubated for one hour at RT. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective. The images displayed HeLa cells during cell cycle progression.)
Immunofluorescence (IF)
(HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with MBS6011004 (1:2500) (0.2ug/ml) (lower) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG incubated for one hour at RT. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective. The images displayed HeLa cells during cell cycle progression.)
Immunofluorescence (IF)
(Untreated HeLa (Panel A) and overnight nocodazoled-treated HeLa (Panel B) were stained with MBS6011004, followed by Alexa Fluor® 488 anti-alpha-tubulin, DyLight™ 594 goat anti-rat-lgG and DAPI.)
Immunofluorescence (IF)
(Untreated HeLa (Panel A) and overnight nocodazoled-treated HeLa (Panel B) were stained with MBS6011004, followed by Alexa Fluor® 488 anti-alpha-tubulin, DyLight™ 594 goat anti-rat-lgG and DAPI.)
NCBI and Uniprot Product Information
Uniprot Description
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Similar Products
Product Notes
The Histone H3 (Catalog #AAA6011004) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, phosphorylated (Ser28) (Histone H3.1, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, H reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC). Not recommended for ChIP (Chromatin Immunoprecipitation) assays. Other applications not tested. Western Blot: 0.5-1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Histone H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
