Immunofluorescence (IF)
(Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded rat brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT. Tissues were then probed with MBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Mouse anti-Human Neurofilament L, 68kD Monoclonal Antibody
Neurofilament L, 68kD (NF-L)
Purified from ascites fluid.
Purified from ascites fluid.
Dilution: ELISA: 0.1-0.5ug/ml
Immunohistochemistry (alcohol and paraformaldehyde-fixed paraffin-embedded or frozen tissue sections): 1-5ug/ml
Western Blot: 0.1-0.5ug/ml
Immunoprecipitation: 2-5ug
Immunofluorescence (IF)
(Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded rat brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT. Tissues were then probed with MBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunofluorescence (IF)
(Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded rat brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT. Tissues were then probed with MBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunofluorescence (IF)
(Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded mouse brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT Tissues were then probed with MBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibod, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunofluorescence (IF)
(Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded mouse brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT Tissues were then probed with MBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibod, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunohistochemistry (IHC)
(Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded human brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT Tissues were then probed withMBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibod, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunohistochemistry (IHC)
(Immunofluorescent analysis of the neurofilament light chain in paraffin-embedded human brain tissue (right) compared to a negative control without MBS608850 (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at RT Tissues were then probed withMBS608850 in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with an IgG (H+L) goat antimMouse secondary antibod, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei ( blue) were stained with DAPI. Images were taken at 40X magnification.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of the neurofilament light chain showing staining in the filaments of paraffin-embedded rat brain tissue (right) compared to a negative control without MBS608850 (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at RT, washed with ddH2O and PBS, and then probed with MBS608850 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of the neurofilament light chain showing staining in the filaments of paraffin-embedded rat brain tissue (right) compared to a negative control without MBS608850 (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate, pH 6.0 and microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at RT, washed with ddH2O and PBS, and then probed with MBS608850 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.)
Western Blot (WB)
(Western Blot analysis of neurofilament, light chain was performed by loading 25ug of human and mouse brain tissue lysate per well onto a polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked. NF-L was detected at ~70kD using MBS608850 at a dilution of 0.5ug/ml in blocking buffer overnight at 4C, followed by a HRP-labeled secondary antibody for 1 hour at RT and detection with a chemiluminescent substrate.)
Western Blot (WB)
(Western Blot analysis of neurofilament, light chain was performed by loading 25ug of human and mouse brain tissue lysate per well onto a polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked. NF-L was detected at ~70kD using MBS608850 at a dilution of 0.5ug/ml in blocking buffer overnight at 4C, followed by a HRP-labeled secondary antibody for 1 hour at RT and detection with a chemiluminescent substrate.)
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Product Notes
The Neurofilament L, 68kD (Catalog #AAA608850) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament L, 68kD (NF-L) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament L, 68kD can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: ELISA: 0.1-0.5ug/ml Immunohistochemistry (alcohol and paraformaldehyde-fixed paraffin-embedded or frozen tissue sections): 1-5ug/ml Western Blot: 0.1-0.5ug/ml Immunoprecipitation: 2-5ug. Researchers should empirically determine the suitability of the Neurofilament L, 68kD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament L, 68kD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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