Mouse anti-Human Podoplanin Monoclonal Antibody | anti-PDPN antibody

Podoplanin

Cat. Num. AAA344076

• Gene Names: PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
• Reactivity: Human
• Synonyms: Podoplanin; Monoclonal Antibody; anti-PDPN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Sequence Length: 162

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Tonsil
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(Podoplanin [IHC650] on Testicular Cancer)

Related Product Information for anti-PDPN antibody

Intended Use: The Podoplanin [IHC650] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Podoplanin is a transmembrane mucoprotein specifically expressed in the endothelium of lymphatic capillaries, while remaining absent from the blood vasculature. The protein is co-localized with VEGFR3/FLT4 in normal skin and kidney. Anti-Podoplanin is useful in the identification of lymphangiomas, Kaposi's sarcomas, epithelioid mesotheliomas, hemangioblastomas, seminomas, and some angiosarcomas which likely have lymphatic differentiation.

References

1. Ordonez NG. Adv Anat Pathol. 2006; 13:83-8.
2. Ordonez NG. Hum Pathol. 2005; 36:372-80.
3. Niakosari F, et al. Arch Dermatol. 2005; 141:440-4.
4. Galambos C, et al. Pediatr Dev Pathol. 2005; 8:191-9.
5. Fukunaga M. Histopathology. 2005; 46:396-402.
6. Fogt F, et al. Oncol Rep. 2004; 11:47-50.
7. Kahn HJ, et al. Mod Pathol. 2002; 15:434-40.
8. Franke FE, et al. J Cutan Pathol. 2004; 31:362-7.
9. Kalof AN,et al. Adv Anat Pathol. 2009; 16:62-4.
10. Chu AY, et al. Mod Pathol. 2005; 18:105-10.
11. Iczkowski KA, et al. Hum Pathol. 2008; 39:275-81.

NCBI and Uniprot Product Information

• NCBI GI #: 21666312
• NCBI GeneID: 10630
• Molecular Weight: 18,173 Da
• NCBI Official Full Name: podoplanin
• NCBI Official Synonym Full Names: podoplanin
• NCBI Official Symbol: PDPN
• NCBI Official Synonym Symbols: T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
• NCBI Protein Information: podoplanin
• UniProt Protein Name: Podoplanin
• Protein Family: Podoplanin
• UniProt Gene Name: PDPN
• UniProt Synonym Gene Names: Gp361 PublicationManual assertion based on opinion iniRef.2; T1ABy similarityManual assertion inferred from sequence similarity toiUniProtKB:Q64294Â (PDPN_RAT); PICD1 PublicationManual assertion based on opinion iniRef.11

NCBI Description

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed:14522983, PubMed:15231832, PubMed:17616532, PubMed:18215137, PubMed:17222411). Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN (PubMed:18541721). Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness (PubMed:17046996, PubMed:21376833). Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (PubMed:20962267). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (). Through binding with LGALS8 may participate to connection of the lymphatic endothelium to the surrounding extracellular matrix (PubMed:19268462). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed:15515019). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (PubMed:25486435). Required for normal lung cell proliferation and alveolus formation at birth (). Does not function as a water channel or as a regulator of aquaporin-type water channels (PubMed:9651190). Does not have any effect on folic acid or amino acid transport ().