Immunohistochemistry (IHC)
(MUC5AC [IHC625] on Stomach Cancer)
Mouse anti-Human MUC5AC Monoclonal Antibody | anti-MUC5AC antibody
MUC5AC
Gene Names
MUC5AC; TBM; leB; MUC5; mucin
Reactivity
Human
Synonyms
MUC5AC; Monoclonal Antibody; anti-MUC5AC antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Sequence Length
5654
Application Notes
The recommended working dilution range is 1:100 - 1:200.
Positive Control
Stomach
Principles and Procedures
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
Reconstitution
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
Specimen Collection and Preparation for Analysis
Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.
To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.
Positive and negative controls should be simultaneously run with unknown specimens.
Immunohistochemistry (IHC)
(MUC5AC [IHC625] on Stomach Cancer)
Immunohistochemistry (IHC)
(MUC5AC [IHC625] on Stomach Cancer)
Related Product Information for anti-MUC5AC antibody
Intended Use: The MUC5AC [IHC625] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Mucin 5AC (MUC5AC) is a secretory-type mucin found in columnar mucous cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon, but not in normal colon cells. MUC5AC expression is indicated in carcinomas wherein the type is defined as diffuse and infiltrative, and those located mainly in the antrum. Studies have also suggested a correlation between MUC5AC and colorectal signet-ring cell carcinoma, with overexpression of MUC5AC relating to the carcinogenesis, malignant potential, progression, and clinical behaviors.
Description: Mucin 5AC (MUC5AC) is a secretory-type mucin found in columnar mucous cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon, but not in normal colon cells. MUC5AC expression is indicated in carcinomas wherein the type is defined as diffuse and infiltrative, and those located mainly in the antrum. Studies have also suggested a correlation between MUC5AC and colorectal signet-ring cell carcinoma, with overexpression of MUC5AC relating to the carcinogenesis, malignant potential, progression, and clinical behaviors.
References
1. Fetsch PA, et al. Cancer. 1998; 84:101-8.
2. Ichihara T, et al. Cancer. 1988; 61:324-33.
3. Soslow RA, et al. Int J Gynecol Pathol. 1996; 15:257-65.
4. Charpin C, et al. Int J Gynecol Pathol. 1982; 1:231-45.
5. Reis CA, et al. Int J Cancer. 1997; 74:112-21.
6. Kim GE, et al. Gastroenterology. 2002; 123:1052-60.
7. Chaves P, et al. Dis Esophagus. 2005; 18:383-7.
8. Leteurtre E, et al. World J Gastroenterol. 2006; 12:3324-31.
9. Mino-Kenudson M, et al. Arch Pathol Lab Med. 2007; 131:86-90.
10.Mizoshita T, et al. Histol Histopathol. 2007; 22:251-60.
11. O'Connell FP, et al. Arch Pathol Lab Med. 2005; 129:338-47.
12. Park SY, et al. Arch Pathol Lab Med. 2007; 131:1561-7.
13. Rakha EA, et al. Mod Pathol. 2005; 18:1295-304.
2. Ichihara T, et al. Cancer. 1988; 61:324-33.
3. Soslow RA, et al. Int J Gynecol Pathol. 1996; 15:257-65.
4. Charpin C, et al. Int J Gynecol Pathol. 1982; 1:231-45.
5. Reis CA, et al. Int J Cancer. 1997; 74:112-21.
6. Kim GE, et al. Gastroenterology. 2002; 123:1052-60.
7. Chaves P, et al. Dis Esophagus. 2005; 18:383-7.
8. Leteurtre E, et al. World J Gastroenterol. 2006; 12:3324-31.
9. Mino-Kenudson M, et al. Arch Pathol Lab Med. 2007; 131:86-90.
10.Mizoshita T, et al. Histol Histopathol. 2007; 22:251-60.
11. O'Connell FP, et al. Arch Pathol Lab Med. 2005; 129:338-47.
12. Park SY, et al. Arch Pathol Lab Med. 2007; 131:1561-7.
13. Rakha EA, et al. Mod Pathol. 2005; 18:1295-304.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
585,570 Da
NCBI Official Full Name
mucin-5AC
NCBI Official Synonym Full Names
mucin 5AC, oligomeric mucus/gel-forming
NCBI Official Symbol
MUC5AC
NCBI Official Synonym Symbols
TBM; leB; MUC5; mucin
NCBI Protein Information
mucin-5AC
UniProt Protein Name
Mucin-5AC
Protein Family
UniProt Gene Name
MUC5AC
UniProt Synonym Gene Names
LeB1 PublicationManual assertion based on opinion iniRef.13; TBM1 PublicationManual assertion based on opinion iniRef.7
Uniprot Description
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
Research Articles on MUC5AC
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Product Notes
The MUC5AC muc5ac (Catalog #AAA344066) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MUC5AC reacts with Human and may cross-react with other species as described in the data sheet. The recommended working dilution range is 1:100 - 1:200. Researchers should empirically determine the suitability of the MUC5AC muc5ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC5AC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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