Typical Testing Data/Standard Curve (for reference only)
Rat NAP-2 (Neutrophil Activating Protein-2) CLIA Kit | NAP-2/CXCL7 clia kit
Rat NAP-2 (Neutrophil Activating Protein-2) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to NAP-2/CXCL7. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NAP-2/CXCL7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NAP-2/CXCL7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of NAP-2/CXCL7. You can calculate the concentration of NAP-2/CXCL7 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. The protein also is an antimicrobial protein with bactericidal and antifungal activity. [provided by RefSeq, Nov 2014]
Uniprot Description
PPBP: LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Secreted, signal peptide; Chemokine; Secreted
Chromosomal Location of Human Ortholog: 4q12-q13
Cellular Component: extracellular region; extracellular space
Molecular Function: chemokine activity; CXCR chemokine receptor binding; glucose transmembrane transporter activity; growth factor activity
Biological Process: blood coagulation; defense response to bacterium; G-protein coupled receptor protein signaling pathway; glucose transport; immune response; inflammatory response; leukocyte migration during inflammatory response; neutrophil chemotaxis; neutrophil degranulation; platelet activation; platelet degranulation; positive regulation of cell division; positive regulation of leukocyte chemotaxis; regulation of cell proliferation; response to lipopolysaccharide
Research Articles on NAP-2/CXCL7
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Product Notes
The Rat NAP-2/CXCL7 ppbp (Catalog #AAA2533001) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533001 CLIA Kit recognizes Rat NAP-2/CXCL7. It is sometimes possible for the material contained within the vial of "NAP-2 (Neutrophil Activating Protein-2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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