Mouse anti-Human MSH2 Monoclonal Antibody | anti-MSH2 antibody

MSH2

Cat. Num. AAA344032

• Reactivity: Human
• Synonyms: MSH2; Monoclonal Antibody; anti-MSH2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 957

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Colon Mucosa, Colon Carcinoma
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(MSH2 [IHC410] on Esophagus)

Related Product Information for anti-MSH2 antibody

Intended Use: The MSH2 [IHC410] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: MutS Homolog 2 (MSH2) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Expression levels of MSH2 are abnormally low in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MSH2 is optimized when paired in an IHC panel with antibodies against MSH6, MLH1, and PMS2. Reports have shown Anti-MSH2 to be useful in the detection of the protein in a number of normal and neoplastic tissues, and for identifying a loss of MSH2 in tumors that are microsatellite-unstable.

References

1. Pal T, et al. Cancer. 2008; 113:733-42.
2. Brueckl WM, et al. Anticancer Res. 2003; 23:1773-8.
3. Rigau V, et al. Arch Pathol Lab Med. 2003; 127:694-700.
4. Renkonen E, et al. J Clin Oncol. 2003; 21:3629-37.
5. Hoedema R, et al. Am Surg. 2003; 69:387-92.
6. Christensen M, et al. Cancer. 2002; 95:2422-30.
7. Wahlberg SS, et al. Cancer Res. 2002; 62:3485-92.
8. Lanza G, et al. Mod Pathol. 2002; 15:741-9.
9. Thibodeau SN, et al. Cancer Res 1996; 56:4836-40.

NCBI and Uniprot Product Information

• NCBI GI #: 1102845313
• Molecular Weight: 107,754 Da
• NCBI Official Full Name: MSH2
• UniProt Protein Name: MSH2
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MSH2

Uniprot Description

Component of the post-replicative DNA mismatch repair system (MMR).