Mouse anti-Human Thyroglobulin Monoclonal Antibody | anti-TG antibody

Thyroglobulin

Cat. Num. AAA344082

• Gene Names: TG; TGN; AITD3
• Reactivity: Human
• Synonyms: Thyroglobulin; Monoclonal Antibody; anti-TG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 2768

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Thyroid
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(Thyroglobulin [IHC674] on Thyroid Cancer)

Related Product Information for anti-TG antibody

Intended Use: The Thyroglobulin [IHC674] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Thyroglobulin is a precursor to the thyroid hormones T4 and T3 and is present in the thyroid follicular cells. Nearly all thyroid follicular carcinomas stain for thyroglobulin and sometimes produce a focal staining pattern. Conversely, poorly differentiated carcinomas and non-thyroid adenocarcinomas do not stain for thyroglobulin, therefore this Thyroglobulin IVD antibody is a useful tool for recognizing papillary and follicular thyroid carcinomas. A panel of Anti-Thyroglobulin and Anti-Calcitonin is useful for identifying medullary thyroid carcinomas, whereas a panel of Anti-Thyroglobulin and Anti-TTF1 is useful for distinguishing between primary thyroid and lung neoplasms.

References

1. Bellet D, et al. J Clin Endocrin Metab. 1983; 56:530-3.
2. Heffess CS, et al. Cancer. 2002; 95:1869-78.
3. Bejarano PA, et al. Appl Immunohistochem Mol Morphol. 2000; 8:189-94.
4. Judkins AR, et al. Hum Pathol. 1999; 30:1373-6.
Hammer SP. Hum Pathol. 1998; 29:1393-402.

NCBI and Uniprot Product Information

• NCBI GI #: 55770862
• NCBI GeneID: 7038
• NCBI Accession #: NP_003226.4
• NCBI GenBank Nucleotide #: NM_003235.4
• UniProt Accession #: P01266; O15274; O43899; Q15593; Q15948; Q9NYR1; Q9NYR2; Q9UMZ0; Q9UNY3
• Molecular Weight: 298,383 Da
• NCBI Official Full Name: thyroglobulin
• NCBI Official Synonym Full Names: thyroglobulin
• NCBI Official Symbol: TG
• NCBI Official Synonym Symbols: TGN; AITD3
• NCBI Protein Information: thyroglobulin
• UniProt Protein Name: Thyroglobulin
• Protein Family: Thyroglobulin
• UniProt Gene Name: TG
• UniProt Synonym Gene Names: Tg

NCBI Description

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]

Uniprot Description

Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).