Mouse anti-Human MSH6 Monoclonal Antibody | anti-MSH6 antibody

MSH6

• Gene Names: Msh6; CG7003; DmelCG7003
• Reactivity: Human
• Synonyms: MSH6; Monoclonal Antibody; anti-MSH6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 1190

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Colon, Colon Carcinoma
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(MSH6 [IHC006] on Esophagus)

Related Product Information for anti-MSH6 antibody

Intended Use: The MSH6 [IHC006] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: MutS Homolog 6 (MSH6) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Studies have shown that mutations in MSH6, when co-indicated with mutations in MSH1 and MSH2, contribute to the development of sporadic colorectal carcinoma. Use of Anti-MSH6 is optimized when paired in an IHC panel with MSH2, MLH1, and PMS2.

References

1. Lagerstedt Robinson K, et al. J Natl Cancer Inst. 2007; 99:291-9.
2. Niessen RC, et al. Gut. 2006; 55:1781-8.
3. Hansen TP, et al. Appl Immunohistochem Mol Morphol. 2006; 14:115-21.
4. Lawes DA, et al. Br J Cancer. 2005; 93:472-7.
5. Stormorken AT, et al. J Clin Oncol. 2005; 23:4705-12.
6. Rigau V, et al. Arch Pathol Lab Med. 2003; 127:694-700.
7. Renkonen E, et al. J Clin Oncol. 2003; 21:3629-37.

NCBI and Uniprot Product Information

• NCBI GI #: 24664545
• NCBI GeneID: 39654
• NCBI Accession #: NP_648755.1
• NCBI GenBank Nucleotide #: NM_140498.2
• UniProt Accession #: Q9VUM0; B6IDV3
• Molecular Weight: 133,136 Da
• NCBI Official Full Name: Msh6
• NCBI Official Symbol: Msh6
• NCBI Official Synonym Symbols: CG7003; DmelCG7003
• NCBI Protein Information: Msh6
• UniProt Protein Name: Probable DNA mismatch repair protein Msh6
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: Msh6

Uniprot Description

Involved in post-replicative DNA-mismatch repair.