Mouse anti-Human PAX-5 Monoclonal Antibody | anti-PAX-5 antibody

PAX-5

Cat. Num. AAA344005

• Gene Names: PAX5; ALL3; BSAP
• Reactivity: Human
• Synonyms: PAX-5; Monoclonal Antibody; anti-PAX-5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 283

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Tonsil
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(PAX-5 [IHC005] on Tonsil)

Related Product Information for anti-PAX-5 antibody

Intended Use: The PAX5 [IHC005] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: PAX-5 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. The PAX-5 gene encodes the B-cell lineage specific activator protein (BSAP), whose expression is limited to early stages of B-cell differentiation. Anti-PAX-5 is useful in differentiating between classic Hodgkin's lymphoma versus multiple myeloma and solitary plasmacytoma, as the protein is expressed in mature and precursor B-cell non-Hodgkin's lymphomas/leukemias while being absent from the other two conditions. Diffuse large B-cell lymphomas are positive for PAX-5, with the exception of those with terminal B-cell differentiation, and T-cell neoplasms do not stain with Anti-PAX-5.

References

1. Torlakovic E, et al. Am J Surg Pathol. 2002; 26:1343-50.
2. Willenbrock K, et al. Lab Invest. 2002; 82:1103-9.
3. Falini B, et al. Blood. 2002; 99:409-26.
4. Schwering I, et al. Blood. 2003; 101:1505-12.

NCBI and Uniprot Product Information

• NCBI GI #: 525507370
• NCBI GeneID: 5079
• NCBI Accession #: NP_001267485.1
• NCBI GenBank Nucleotide #: NM_001280556.1
• UniProt Accession #: Q02548; O75933; Q5SFM2; Q6S728; A3QVP6; A3QVP7; A3QVP8; C0KTF6; C0KTF7; C0KTF8; C0KTF9; C0KTG0
• Molecular Weight: 38,890 Da
• NCBI Official Full Name: paired box protein Pax-5 isoform 11
• NCBI Official Synonym Full Names: paired box 5
• NCBI Official Symbol: PAX5
• NCBI Official Synonym Symbols: ALL3; BSAP
• NCBI Protein Information: paired box protein Pax-5
• UniProt Protein Name: Paired box protein Pax-5
• UniProt Gene Name: PAX5
• UniProt Synonym Gene Names: BSAP

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.