Mouse anti-Human Cytokeratin 18 Monoclonal Antibody | anti-KRT18 antibody

Cytokeratin 18

Cat. Num. AAA344011

• Gene Names: KRT18; K18; CK-18; CYK18
• Reactivity: Human
• Synonyms: Cytokeratin 18; Monoclonal Antibody; anti-KRT18 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 430

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Breast, Breast Carcinoma
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(Cytokeratin 18 [IHC018] on Colon - 10X)

Related Product Information for anti-KRT18 antibody

Intended Use: The Cytokeratin 18 [IHC018] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Cytokeratin 18 (CK18) is present in simple, glandular, and transitional epithelial cells, but is absent in stratified epithelial cells. CK18 usually multimerizes with Cytokeratin 8, and Anti-Cytokeratin 18 is useful for detecting adenocarcinomas of simple and glandular epithelium origin, as well as poorly differentiated squamous carcinoma cells.

References

1. Moll R. Subcell Biochem. 1998; 31:205-62.
2. Ku NO, et al. J Clin Invest. 1997; 99:19-23.
3. Woelfle U, et al. Clin Cancer Res. 2004; 10:2670

NCBI and Uniprot Product Information

• NCBI GI #: 34037
• NCBI GeneID: 3875
• UniProt Accession #: P05783; Q53G38; Q5U0N8; Q9BW26
• Molecular Weight: 48,058 Da
• NCBI Official Full Name: cytokeratin 18
• NCBI Official Synonym Full Names: keratin 18
• NCBI Official Symbol: KRT18
• NCBI Official Synonym Symbols: K18; CK-18; CYK18
• NCBI Protein Information: keratin, type I cytoskeletal 18
• UniProt Protein Name: Keratin, type I cytoskeletal 18
• Protein Family: Keratin
• UniProt Gene Name: KRT18
• UniProt Synonym Gene Names: CYK18; CK-18; K18

NCBI Description

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.