Western Blot (WB)
(Western Blot using anti-Lewis a,b antibody HEA164 (MBS488694) Human colorectal carcinoma tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HEA164 () at 0.001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rabbit anti-Human Lewis a,b Monoclonal Antibody
Anti-Lewis a,b [HEA164]
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation
Purity
Purified antibody. Protein A affinity purified
Synonyms
Lewis a; b; Monoclonal Antibody; Anti-Lewis a; b [HEA164]; Blood Group Lewis a/b; lewis a; lewis b; anti-Lewis a; b antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
HEA164
Specificity
This antibody is specific for Lewis a,b carbohydrate antigens.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Lewis a,b antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
This antibody has applications in the detection of normal and neoplastic epithelial cells. This antibody has been shown to react with a glycolipid fraction isolated from colon carcinoma tissue, with no effect of pronase E addition on its reactivity (Moldenhauer et al, 1987). This antibody, when immobilised on the wall of a shear chamber, has been shown to support slow leukocyte rolling (Chen et al, 1997).
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
PMID
2449234
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Western Blot (WB)
(Western Blot using anti-Lewis a,b antibody HEA164 (MBS488694) Human colorectal carcinoma tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HEA164 () at 0.001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-Lewis a,b antibody HEA164 (MBS488694) Human colorectal carcinoma tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HEA164 () at 0.001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Caco-2 cells with anti-Lewis a,b antibody HEA164 (MBS488694) Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of HEA164 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Caco-2 cells with anti-Lewis a,b antibody HEA164 (MBS488694) Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of HEA164 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-Lewis a,b antibody
Recombinant monoclonal antibody to Lewis a,b. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HEA164.
This antibody was raised by immunising mice with human carcinoma cells.
This antibody was raised by immunising mice with human carcinoma cells.
Product Categories/Family for anti-Lewis a,b antibody
References
Moldenhauer et al. Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br J Cancer. 1987 Dec; 56(6): 714-721.
Note on publication: Describes the original use of this antibody.
Note on publication: Describes the original use of this antibody.
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Product Notes
The Lewis a,b (Catalog #AAA488695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Lewis a,b [HEA164] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lewis a,b can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP). This antibody has applications in the detection of normal and neoplastic epithelial cells. This antibody has been shown to react with a glycolipid fraction isolated from colon carcinoma tissue, with no effect of pronase E addition on its reactivity (Moldenhauer et al, 1987). This antibody, when immobilised on the wall of a shear chamber, has been shown to support slow leukocyte rolling (Chen et al, 1997). Researchers should empirically determine the suitability of the Lewis a,b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lewis a,b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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