Mouse anti-Human p53 Monoclonal Antibody | anti-p53 antibody

p53

Cat. Num. AAA344017

• Gene Names: TP53; P53; BCC7; LFS1; TRP53
• Reactivity: Human
• Synonyms: p53; Monoclonal Antibody; anti-p53 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 393

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Colon Carcinoma
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(p53 [IHC053] on Colon Cancer)

Related Product Information for anti-p53 antibody

Intended Use: The p53 [IHC053] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: p53, also known as tumor protein 53 or TP53, is a tumor suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumors, including those of the breast, ovarian, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.

References

1. Stoehlmacker J, et al. Oncol. 2003; 30:10-16.
2. Gallo O, et al. Hum Pathol. 2002; 33:708-714.
3. Sano H, et al. Cancer Res. 1995: 55:3785-9.
4. Denkert C, et al. Cancer. 2003; 97:2978-87.
5. Sheehan KM, et al. Hum Pathol. 2003; 34:1242-1246.
6. Moore BE, et al. Appl Immunohistochem Mol Morphol. 2001; 9:203-6.
7. Mauri FA, et al. Int J Oncol. 1999; 15:1137-47.
8. Caffo O, et al. Clin Cancer Res. 1996; 2:1591-9.
9. Bebenek M, et al. Anticancer Res. 1998; 18:619-23.
10. Midulla C, et al. Anticancer Res. 1999; 19:4033-7.
11. Zen ZS, et al. J Clin Oncol. 1994; 12:2043-50.
12. Quinlan DC, et al. Cancer Res. 1992; 53:4828-31.
13. Alexiev BA, et al. Gen Diagn Pathol. 1997; 142:271-9.

NCBI and Uniprot Product Information

• NCBI GI #: 23491729
• NCBI GeneID: 7157
• Molecular Weight: 37,826 Da
• NCBI Official Full Name: P53
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53
• UniProt Protein Name: Cellular tumor antigen p53
• Protein Family: p53 and DNA damage-regulated protein
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53

NCBI Description

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Uniprot Description

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).