Mouse anti-Human CD2 Monoclonal Antibody | anti-CD2 antibody

CD2

Cat. Num. AAA344044

• Reactivity: Human
• Synonyms: CD2; Monoclonal Antibody; anti-CD2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 181

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Tonsil
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(CD2 [IHC531] on Tonsil)

Related Product Information for anti-CD2 antibody

Intended Use: The CD2 [IHC531] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Cluster of differentiation 2 (CD2) is a useful early T-cell lineage restricted antigen that is present in T-cell differentiation. As a pan-T-cell marker, CD2 staining is used for recognizing practically all normal T-cells, but may be deleted in some T-cell neoplasms. Since CD2 is present in most precursor and mature T-cell leukemias and lymphomas, it is useful in the evaluation of lymphoid malignancies. By using CD2 and CD25 staining, the recognition of systemic mastocytosis and mastocytic leukemia is supported.

References

1. Went P, et al. J Clin Oncol. 2006; 24:2472-2479.
2. Aguilera NS, et al. Arch Pathol Lab Med. 2006; 130:1772-9.
3. Barrionuevo C, et al. Appl Immunohistochem Mol Morphol. 2007; 15:38-44.
4. Bovenschen HJ, et al. Br J Dermatol. 2005; 153:72-8.
5. Foon KA, et al. Blood 1986; 68:1- 31.

NCBI and Uniprot Product Information

• NCBI GI #: 958931301
• Molecular Weight: 19,842 Da
• NCBI Official Full Name: CD2, partial
• UniProt Protein Name: CD2
• Protein Family: CD2
• UniProt Gene Name: CD2