Mouse anti-Human Kappa Monoclonal Antibody | anti-Kappa antibody

Kappa

Cat. Num. AAA344062

• Reactivity: Human
• Synonyms: Kappa; Monoclonal Antibody; anti-Kappa antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 107

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Tonsil
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(Kappa [IHC610] on Ovary)

Related Product Information for anti-Kappa antibody

Intended Use: The Kappa [IHC610] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Anti-Kappa recognizes surface immunoglobulin on normal and neoplastic B-cells, and has been indicated as a potential aid in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas, where the expression of a single light chain class is restricted. The determination of light chain ratio is critical in evaluating B-cell neoplasms, as the majority of B-cell lymphomas express either kappa or lambda light chains, while a mixture of kappa and lambda is characteristic of reactive proliferations. In paraffin-embedded tissue, Anti-Kappa displays strong staining of kappa-positive plasma cells, as well as cells that have absorbed exogenous immunoglobulins.

References

1. Bray M, et al. Am J Clin Pathol. 1983; 80:526-8.
2. Ashton-Key M, et al. Histopathology. 1996; 29:525-31.
3. Kurtin PJ, et al. Am J Clin Pathol. 1999; 112:319-29.
4. Falini B, et al. J Histochem Cytochem. 1982; 30:21-6.
5. Marshall-Taylor CE, et al. Appl Immunohistochem Mol Morphol. 2002; 10:258-62.
6. Sobol RE, et al. Clin Immunol Immunopathol. 1982; 24:139 -44.
7. Samoszuk MK, et al. Diagn Immunol. 1985; 3:133-8.
8. Abbondanzo SL, et al. Ann Diagn Pathol. 1999; 3:318-27.

NCBI and Uniprot Product Information

• NCBI GI #: 2627250
• Molecular Weight: 11,933 Da
• NCBI Official Full Name: kappa, partial
• UniProt Protein Name: Kappa
• Protein Family: Kappa-casein
• UniProt Gene Name: kappa