Mouse Neurofilament H Monoclonal Antibody | anti-AT5G64690 antibody

Neurofilament H, phosphorylated (Neurofilament Heavy Polypeptide, NF-H, Neurofilament Triplet H Protein, 200kD Neurofilament Protein, NEFH, NFH)

Cat. Num. AAA6012857

• Gene Names: AT5G64690; MUB3.22; MUB3_22
• Reactivity: Human, Porcine, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: Neurofilament H; Monoclonal Antibody; phosphorylated (Neurofilament Heavy Polypeptide; NF-H; Neurofilament Triplet H Protein; 200kD Neurofilament Protein; NEFH; NFH); Anti -Neurofilament H; anti-AT5G64690 antibody

• Host: Mouse
• Reactivity: Human, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 13B840
• Specificity: Recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some crossreactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. Recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-AT5G64690 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.; Dilution: Immunofluorescence: 1:1000; Immunohistochemistry: Formalin-fixed sections and frozen sections in tissue culture.; Western Blot: 1:10,000
• Immunogen: Preparation of native NF-H purified from porcine spinal cord.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including MBS6012857 (lane 2).)

Immunohistochemistry (IHC)

(Human cerebellar cortex fixed in formalin, embedded in paraffin and stained with MBS6012857 using the ABC (avidin biotin conjugate) method. The section was counterstained with heamatoxylin-eosin (blue). MBS6012857 stains prominent basket cell axons surrounding the large Purkinje neurons. Granule cell layer at bottom of image, molecular layer at top.)

Related Product Information for anti-AT5G64690 antibody

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (HUGO names are NEFL, NEFM and NEFH respectively). NF-H is high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kD, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. 031397 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with 031397 in dendritic and perikaryal neurofilaments. The HGNC name for this protein is NEFH.

Product Categories/Family for anti-AT5G64690 antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 15237758
• NCBI GeneID: 836590
• NCBI Accession #: NP_201274.1
• NCBI GenBank Nucleotide #: NM_125865.3
• UniProt Accession #: Q9FGG4
• Molecular Weight: 38,144 Da
• NCBI Official Full Name: neurofilament triplet H protein-like protein
• NCBI Official Symbol: AT5G64690
• NCBI Official Synonym Symbols: MUB3.22; MUB3_22
• NCBI Protein Information: neurofilament triplet H protein-like protein
• UniProt Protein Name: At5g64690
• Protein Family: Neurofilament heavy polypeptide
• UniProt Entry Name: Q9FGG4_ARATH