Mouse Neurofilament NF-H Monoclonal Antibody | anti-NF-H antibody

Neurofilament NF-H

Cat. Num. AAA556085

• Gene Names: NEFH; NFH
• Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
• Applications: Immunocytochemistry, Western Blot, Immunohistochemistry, Immunofluorescence
• Synonyms: Neurofilament NF-H; Monoclonal Antibody; anti-NF-H antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Form/Format: Liquid; Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

• Applicable Applications for anti-NF-H antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IF: 1:1,000; IHC: 1:1,000; ICC: 1:1,000; WB: 1:10,000
• Immunogen: Native NF-H purified from bovine spinal cord
• Preparation and Storage: Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2-8 degree C for 1 month without detectable loss ofactivity. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistological analysis of rat cerebellum section stained with mouse mAb to pNF-H, MO22187, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000 in red. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies.)

Western Blot (WB)

(Western blot detection of the heavily phosphorylated axonal form of NF-H protein (pNF-H) in neural tissue lysates (20ug/lane) with affinity purified mouse monoclonal anti-pNF-H antibody at dilution of 1:5,000. Lanes on the blot are: [1] Protein size marker, [2] Adult rat whole brain [3] Embryonic (E20) rat whole brain [4] Adult rat spinal cord [5] Adult mouse whole brain [6] Adult mouse spinal cord.)

Related Product Information for anti-NF-H antibody

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other proteins may also be present. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying axonal processes in tissue sections and in culture. NF-H antibodies can also be useful in visualizing neurofilament accumulations seen in many neurological disorders, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig's disease), Alzheimer's disease and following a traumatic injury.

NCBI and Uniprot Product Information

• NCBI GI #: 32483416
• NCBI GeneID: 4744
• NCBI Accession #: NP_066554.2
• NCBI GenBank Nucleotide #: NM_021076.3
• UniProt Accession #: P12036; Q96HF8; Q9UJS7; Q9UQ14; B4DYY4
• Molecular Weight: 112,479 Da
• NCBI Official Full Name: neurofilament heavy polypeptide
• NCBI Official Synonym Full Names: neurofilament, heavy polypeptide
• NCBI Official Symbol: NEFH
• NCBI Official Synonym Symbols: NFH
• NCBI Protein Information: neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa
• UniProt Protein Name: Neurofilament heavy polypeptide
• UniProt Gene Name: NEFH
• UniProt Synonym Gene Names: KIAA0845; NFH; NF-H
• UniProt Entry Name: NFH_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

NFH: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: mitochondrion; axon; cytoplasm; neurofilament

Molecular Function: protein binding, bridging; dynein binding; structural constituent of cytoskeleton; kinesin binding; microtubule binding; structural molecule activity; protein kinase binding

Biological Process: axonogenesis; neurofilament bundle assembly; microtubule cytoskeleton organization and biogenesis; cell projection biogenesis; peripheral nervous system neuron axonogenesis

Disease: Amyotrophic Lateral Sclerosis 1