Typical Testing Data/Standard Curve (for reference only)
Monkey Osteocalcin ELISA Kit | OC/BGP elisa kit
Monkey OC/BGP (Osteocalcin) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey OC/BGP. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Monkey OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Monkey OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey OC/BGP. The concentration of Monkey OC/BGP in samples can be calculated by comparing the OD of the samples with the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
Uniprot Description
osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.
Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular space; Golgi lumen; perikaryon; rough endoplasmic reticulum
Molecular Function: calcium ion binding; hydroxyapatite binding; structural constituent of bone; structural molecule activity
Biological Process: bone mineralization; cell adhesion; cell aging; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; odontogenesis; osteoblast development; osteoblast differentiation; peptidyl-glutamic acid carboxylation; post-translational protein modification; regulation of bone mineralization; regulation of bone resorption; regulation of osteoclast differentiation; response to activity; response to drug; response to estrogen stimulus; response to ethanol; response to glucocorticoid stimulus; response to gravity; response to hydroxyisoflavone; response to mechanical stimulus; response to testosterone stimulus; response to vitamin D; response to vitamin K; response to zinc ion; signal peptide processing; skeletal development
Research Articles on OC/BGP
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Product Notes
The Monkey OC/BGP bglap (Catalog #AAA2533624) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533624 ELISA Kit recognizes Monkey OC/BGP. It is sometimes possible for the material contained within the vial of "Osteocalcin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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