Rabbit anti-Porcine, Rat Neurofilament L, 68kD Polyclonal Antibody

Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)

Cat. Num. AAA6012347

• Reactivity: Porcine, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Serum; Serum
• Synonyms: Neurofilament L; 68kD; Polyclonal Antibody; 68kD (NF-L; NFL; 68kD Neurofilament Protein; NEFL; Neurofilament Light Polypeptide 68kD; NF68; Neurofilament Triplet L Protein); Anti -Neurofilament L; anti-Neurofilament L; 68kD antibody

• Host: Rabbit
• Reactivity: Porcine, Rat
• Clonality: Polyclonal
• Specificity: Recognizes porcine and rat NF-L.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-Neurofilament L, 68kD antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot.; Dilution: Immunocytochemistry (ABC): 1:5000; Immunofluorescence: 1:500-1:1000; Western Blot: 1:20,000
• Immunogen: Porcine intermediate filaments were prepared from spinal cords by the method of Delacourte et al., and this cytoskeletal material was dissolved in 6M urea.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of whole rat cerebellum homogenate stained with MBS6012347 in the left strip, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kD corresponds to rodent NF-L. Faint bands below this are in vivo degradation products generated by calpain digestion. The major NF-H band in human, cow and bovine run a little slower on SDS-PAGE, at about 70kD. Right lane shows a strip incubated with a new antibody against neuron specific enolase, which reveals a major band at 47kD.)

Western Blot (WB)

(Mixed neuron/glia cultures from newborn rat brain stained with 031420 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L MBS6012347 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA)

Related Product Information for anti-Neurofilament L, 68kD antibody

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which include the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include other intermediate filamant subunits such as -internexin, peripherin and in developing neurons nestin and vimentin. Antibodies to neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable and found only in neurons. Mutations in the protein coding region of the human NF-L gene appear to cause some forms of Charcot-Marie-Tooth disease (2) The HGNC name for this protein is NEFL.

Product Categories/Family for anti-Neurofilament L, 68kD antibody

Antibodies; Abs to Neurofilament