Chicken Neurofilament H, 200kD Polyclonal Antibody

Neurofilament H, 200kD (NF-H, NFH, 200kD Neurofilament Protein, KIAA0845, NEFH, Neurofilament Heavy Polypeptide 200kD, Neurofilament Heavy Polypeptide, Neurofilament Triplet H Protein, NF200)

Cat. Num. AAA627970

• Reactivity: Bovine, Feline, Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Serum; Serum
• Synonyms: Neurofilament H; 200kD; Polyclonal Antibody; 200kD (NF-H; NFH; 200kD Neurofilament Protein; KIAA0845; NEFH; Neurofilament Heavy Polypeptide 200kD; Neurofilament Heavy Polypeptide; Neurofilament Triplet H Protein; NF200); Anti -Neurofilament H; anti-Neurofilament H; 200kD antibody

• Host: Chicken
• Reactivity: Bovine, Feline, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes bovine Neurofilament H. Species Crossreactivity: Human, mouse, rat, and cat. Other species have not been tested.
• Purity/Purification: Serum; Serum

• Applicable Applications for anti-Neurofilament H, 200kD antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.
• Immunogen: Purified by NF-H protein from Bovine intermediate filaments prepared from spinal cords.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50%glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunocytochemistry (ICC)

(IC/IF analysis of mixed rat neuron/glial cultures using MBS627970 (red) and an an†ibody against NF-L (green). MBS627970 binds primarily to the phosphorylated axonal forms of NF-H. The surrounding axonal profiles are orange due to staining by both NF-H and NF-L antibodies.)

Immunocytochemistry (ICC)

(IC/IF analysis of rat neurons using MBS627970.)

Immunocytochemistry (ICC)

(IC/IF analysis of SH-SY5Y cells using MBS627970 (red) and fibrillarin antibody (green). Nuclear DNA has been stained with Hoechst dye (blue).)

Western Blot (WB)

(WB analysis of rat spinal cord extract: Lane 1. Coomassie blue staining of electrophoresced extract Lane 2. Extract probed with MBS627970)

Related Product Information for anti-Neurofilament H, 200kD antibody

Neurofilaments can be defined as the intermediate or 10nm filaments found specifically in neuronal cells. Neurofilaments appears as fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid axon impulse conduction. They are composed of a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Product Categories/Family for anti-Neurofilament H, 200kD antibody

Antibodies; Abs to Neurofilament