Mouse Neurofilament L, 68kD Monoclonal Antibody

Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)

Cat. Num. AAA646248

• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: Neurofilament L; 68kD; Monoclonal Antibody; 68kD (NF-L; NFL; 68kD Neurofilament Protein; NEFL; Neurofilament Light Polypeptide 68kD; NF68; Neurofilament Triplet L Protein); Anti -Neurofilament L; anti-Neurofilament L; 68kD antibody

• Host: Mouse
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 13B842
• Specificity: Recognizes NF-L from human, bovine, porcine, mouse, rat and all other mammals.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-Neurofilament L, 68kD antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot.; Dilution: Immunofluorescence: 1:100-1:500; Immunohistochemistry: Frozen and mildly formalin-fixed sections; Western Blot: 1:5000-1:10,000
• Immunogen: Preparation of porcine neurofilament NF-H and NF-L
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Strip blots of rat spinal cord homogenate probed with two sublones of MBS646248. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68kD with essentially no background.)

Testing Data

(Embryonic rat cortical cells grown in tissue culture and stained with MBS646248 (green) and the phosphorylated axonal forrm of neurofilament NF-H, using 031399 antibody (red). The perikaryal and dendritic neurofilaments in the Large cell in middle are stained with MBS646248 but not with the phosphorylated NF-H antibody. In contrast both antibodies stain axonal neurofilaments which therefore appear orange.)

Related Product Information for anti-Neurofilament L, 68kD antibody

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kD. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease and Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (1).

Product Categories/Family for anti-Neurofilament L, 68kD antibody

Antibodies; Abs to Neurofilament