Mouse Neurofilament L, 68kD Monoclonal Antibody

Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)

Cat. Num. AAA6007622

• Reactivity: Avian, Bovine, Human, Mammal, Mouse, Porcine, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: Neurofilament L; 68kD; Monoclonal Antibody; 68kD (NF-L; NFL; 68kD Neurofilament Protein; NEFL; Neurofilament Light Polypeptide 68kD; NF68; Neurofilament Triplet L Protein); Anti -Neurofilament L; anti-Neurofilament L; 68kD antibody

• Host: Mouse
• Reactivity: Avian, Bovine, Human, Mammal, Mouse, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 13B841
• Specificity: Recognizes porcine NF-L. Species Crossreactivity: human, bovine, mouse, rat, avian and all other mammals.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-Neurofilament L, 68kD antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot.; Dilution: Immunofluorescence: 1:100-1:500; Immunohistochemistry: Frozen and formalin fixed sections; Western Blot: 1:5000-1:10,000
• Immunogen: Enzymatically dephosphorylated porcine neurofilaments including NF-L, NF-M and NF-H.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including MBS6007622 (lane 4).)

Testing Data

(Cells grown from adult rat brain using method described by Evans et al. (2). Large cell in middle is stained with MBS6007622 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin 031378 (red). These cells were mitotic but had several characteristics of neurons.)

Related Product Information for anti-Neurofilament L, 68kD antibody

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is light or low molecular weight polypeptide and runs on SDS-PAGE gels at ~68kD. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease and Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease.

Product Categories/Family for anti-Neurofilament L, 68kD antibody

Antibodies; Abs to Neurofilament