Western Blot (WB)
(MBD1 antibody tested by Western blot. MBD1 detection by Western blot. The analysis was performed using HeLa whole-cell extract and MBD1 antibody at 2 ug/ml (lane 1) and a 1 ug/ml (lane 2) dilutions.)
Rabbit anti-Human MBD1 Monoclonal Antibody | anti-MBD1 antibody
MBD1 antibody (mAb)(Clone 100B272.1)
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
MBD1; Monoclonal Antibody; MBD1 antibody (mAb)(Clone 100B272.1); DPEP1; MDP; RDP; anti-MBD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
100B272.1
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-MBD1 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This MBD1 antibody was raised against a synthetic peptide corresponding to human MBD1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(MBD1 antibody tested by Western blot. MBD1 detection by Western blot. The analysis was performed using HeLa whole-cell extract and MBD1 antibody at 2 ug/ml (lane 1) and a 1 ug/ml (lane 2) dilutions.)
Western Blot (WB)
(MBD1 antibody tested by Western blot. MBD1 detection by Western blot. The analysis was performed using HeLa whole-cell extract and MBD1 antibody at 2 ug/ml (lane 1) and a 1 ug/ml (lane 2) dilutions.)
Related Product Information for anti-MBD1 antibody
Short Description: Our MBD1 antibody (mAb)(Clone 100B272.1) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: MBD1 (methyl-CpG binding domain protein 1) is thought to function as a mediator of the biological consequences of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD1 is recruited to both methylated and non-methylated CpGs via separate domains: the CXXC domain targets the protein to non-methylated CpGs whereas the MBD domain targets the protein to methylated CpG. During S phase of the cell cycle, MBD1 can interact with SETDB1 (H3K9 methyltransferase).
Background: MBD1 (methyl-CpG binding domain protein 1) is thought to function as a mediator of the biological consequences of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD1 is recruited to both methylated and non-methylated CpGs via separate domains: the CXXC domain targets the protein to non-methylated CpGs whereas the MBD domain targets the protein to methylated CpG. During S phase of the cell cycle, MBD1 can interact with SETDB1 (H3K9 methyltransferase).
Product Categories/Family for anti-MBD1 antibody
NCBI and Uniprot Product Information
Molecular Weight
70kDa
Protein Family
Similar Products
Product Notes
The MBD1 (Catalog #AAA388411) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MBD1 antibody (mAb)(Clone 100B272.1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MBD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the MBD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
