Western Blot (WB)
(HDAC5 antibody tested by Western blot. Detection of HDAC5 by Western blot. NIH/3T3 cell extract was blotted and probed with HDAC5 antibody at a 2 ug/ml dilution.)
Rabbit anti-Human, Mouse HDAC5 Polyclonal Antibody | anti-HDAC5 antibody
HDAC5 antibody (pAb)
Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
HDAC5; Polyclonal Antibody; HDAC5 antibody (pAb); HD5; NY-CO-9; anti-HDAC5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-HDAC5 antibody
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This HDAC5 antibody was raised against a synthetic peptide corresponding to amino acid residues 572-587 of human HDAC5.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(HDAC5 antibody tested by Western blot. Detection of HDAC5 by Western blot. NIH/3T3 cell extract was blotted and probed with HDAC5 antibody at a 2 ug/ml dilution.)
Western Blot (WB)
(HDAC5 antibody tested by Western blot. Detection of HDAC5 by Western blot. NIH/3T3 cell extract was blotted and probed with HDAC5 antibody at a 2 ug/ml dilution.)
Related Product Information for anti-HDAC5 antibody
Short Description: Our HDAC5 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Immunoprecipitation and Western blot; it has been shown to react with Human and Mouse samples.
Background: HDAC5 (Histone Deacetylase 5) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Like HDAC4, HDAC5 shuttles between the nucleus and cytoplasm. In muscle cells, this protein shuttles into the cytoplasm during myocyte differentiation. HDAC5 interacts with many different transcription factors including HDAC3, and may form multi-complex proteins.
Background: HDAC5 (Histone Deacetylase 5) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Like HDAC4, HDAC5 shuttles between the nucleus and cytoplasm. In muscle cells, this protein shuttles into the cytoplasm during myocyte differentiation. HDAC5 interacts with many different transcription factors including HDAC3, and may form multi-complex proteins.
Product Categories/Family for anti-HDAC5 antibody
NCBI and Uniprot Product Information
Molecular Weight
120kDa
Similar Products
Product Notes
The HDAC5 (Catalog #AAA388707) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HDAC5 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC5 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the HDAC5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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