Western Blot (WB)
(TLR3 mAb tested by Western blot. TLR3 detection by Western blot. The analysis of TLR3 was performed using TLR3 mAb and nuclear extract from 293 cells (lane 1) and nuclear extract from 293 cells that had been transfected with human TLR3 cDNA (lane 2).)
Mouse anti-Human TLR3 Monoclonal Antibody | anti-TLR3 antibody
TLR3 antibody (mAb)(Clone 40C1285)
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
TLR3; Monoclonal Antibody; TLR3 antibody (mAb)(Clone 40C1285); CD283; IIAE2; anti-TLR3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
40C1285
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-TLR3 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This TLR3 antibody was raised against a KLH-conjugated synthetic peptide corresponding to human TLR3.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(TLR3 mAb tested by Western blot. TLR3 detection by Western blot. The analysis of TLR3 was performed using TLR3 mAb and nuclear extract from 293 cells (lane 1) and nuclear extract from 293 cells that had been transfected with human TLR3 cDNA (lane 2).)
Western Blot (WB)
(TLR3 mAb tested by Western blot. TLR3 detection by Western blot. The analysis of TLR3 was performed using TLR3 mAb and nuclear extract from 293 cells (lane 1) and nuclear extract from 293 cells that had been transfected with human TLR3 cDNA (lane 2).)
Related Product Information for anti-TLR3 antibody
Short Description: Our TLR3 antibody (mAb)(Clone 40C1285) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: TLR3-Toll-Like receptor 3 is a member of the Toll-like receptor (TLR) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize specific molecular features associated with pathogens, and mediate the production of cytokines necessary for the development of effective immunity. Many of the TLRs heterodimerize with each other to modulate receptor function. TLR3 expression is increased after exposure to Gram-negative bacteria and to an even greater extent in response to Gram-positive bacteria. TLR3 forms a homodimer and recognizes viral double stranded RNA (dsRNA).
Background: TLR3-Toll-Like receptor 3 is a member of the Toll-like receptor (TLR) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize specific molecular features associated with pathogens, and mediate the production of cytokines necessary for the development of effective immunity. Many of the TLRs heterodimerize with each other to modulate receptor function. TLR3 expression is increased after exposure to Gram-negative bacteria and to an even greater extent in response to Gram-positive bacteria. TLR3 forms a homodimer and recognizes viral double stranded RNA (dsRNA).
Product Categories/Family for anti-TLR3 antibody
NCBI and Uniprot Product Information
Molecular Weight
120kDa
Protein Family
Similar Products
Product Notes
The TLR3 (Catalog #AAA388695) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TLR3 antibody (mAb)(Clone 40C1285) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TLR3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the TLR3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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