Western Blot (WB)
(DRAK2 pAb tested by Western blot. Detection of DRAK2 by Western blot. The analysis was performed using 30 ug HeLa whole-cell extract and DRAK2 pAb at a 2 ug/ml dilution.)
Rabbit anti-Human DRAK2 Polyclonal Antibody | anti-DRAK2 antibody
DRAK2 antibody (pAb)
Gene Names
STK17B; DRAK2
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
DRAK2; Polyclonal Antibody; DRAK2 antibody (pAb); STK17B; anti-DRAK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-DRAK2 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This DRAK2 antibody was raised against a peptide corresponding to amino acid residues 351-365 of human DRAK2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(DRAK2 pAb tested by Western blot. Detection of DRAK2 by Western blot. The analysis was performed using 30 ug HeLa whole-cell extract and DRAK2 pAb at a 2 ug/ml dilution.)
Western Blot (WB)
(DRAK2 pAb tested by Western blot. Detection of DRAK2 by Western blot. The analysis was performed using 30 ug HeLa whole-cell extract and DRAK2 pAb at a 2 ug/ml dilution.)
Related Product Information for anti-DRAK2 antibody
Short Description: Our DRAK2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: Overexpression of DRAK2 (STK17B or DAP kinase-related apoptosis-inducing protein kinase 2) and ZIP kinase can cause the morphological changes typical of apoptosis in NIH/3T3 cells. Both DRAKs and ZIP kinase share significant homology at the amino acid level. The kinase domains of both DRAKs and ZIP kinase are homologous to DAP (Death-associated protein) kinase, which is involved in the apoptotic signaling induced by interferon-g. Overexpression of DRAKs in NIH/3T3 cells lead to apoptosis. These experiments also indicate that the C-terminal domain of DRAKs are important for induction of apoptosis.
Background: Overexpression of DRAK2 (STK17B or DAP kinase-related apoptosis-inducing protein kinase 2) and ZIP kinase can cause the morphological changes typical of apoptosis in NIH/3T3 cells. Both DRAKs and ZIP kinase share significant homology at the amino acid level. The kinase domains of both DRAKs and ZIP kinase are homologous to DAP (Death-associated protein) kinase, which is involved in the apoptotic signaling induced by interferon-g. Overexpression of DRAKs in NIH/3T3 cells lead to apoptosis. These experiments also indicate that the C-terminal domain of DRAKs are important for induction of apoptosis.
Product Categories/Family for anti-DRAK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44.7 kDa (395aa), confirmed by MALDI-TOF
NCBI Official Full Name
serine/threonine-protein kinase 17B
NCBI Official Synonym Full Names
serine/threonine kinase 17b
NCBI Official Symbol
STK17B
NCBI Official Synonym Symbols
DRAK2
NCBI Protein Information
serine/threonine-protein kinase 17B
UniProt Protein Name
Serine/threonine-protein kinase 17B
UniProt Gene Name
STK17B
UniProt Synonym Gene Names
DRAK2
UniProt Entry Name
ST17B_HUMAN
Uniprot Description
DRAK2: a calmodulin-dependent kinase of the DAPK family. Interacts with and is regulated by calcineurin homologous protein (CHP), an EF-hand Ca(2+)-binding protein. In vitro, CHP inhibits the apoptosis-inducing protein kinase DRAK2 in the presence of elevated Ca(2+) levels. Highly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.
Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; DAPK family
Chromosomal Location of Human Ortholog: 2q32.3
Cellular Component: plasma membrane; ER-Golgi intermediate compartment; nucleus; actin cytoskeleton
Molecular Function: protein serine/threonine kinase activity; ATP binding; protein kinase activity
Biological Process: apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation
Research Articles on DRAK2
Similar Products
Product Notes
The DRAK2 stk17b (Catalog #AAA388683) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DRAK2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DRAK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the DRAK2 stk17b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DRAK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
