Rabbit anti-Human, Mouse MBD1 Polyclonal Antibody | anti-MBD1 antibody

MBD1 Antibody

Cat. Num. AAA841423

• Gene Names: MBD1; RFT; PCM1; CXXC3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: MBD1; Polyclonal Antibody; MBD1 Antibody; CXXC-type zinc finger protein 3; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1; anti-MBD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti- MBD1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 655

• Applicable Applications for anti-MBD1 antibody: Western Blot (WB)
• Application Notes: Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Cross activity to perforin of other species has not been determined.
• Immunogen: Synthetic peptide surrounding amino acid 594 of human MBD1
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-MBD1 antibody

Background: The MBD family of proteins includes MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD1 (methyl-CpG binding domain protein 1) binds to methylated CpG sites and represses gene transcription. MBD1 is recruited to both methylated and non-methylated CpGs by separate domains: the CXXC domain targets the protein to non-methylated CpGs whereas the MBD domain targets the protein to methylated CpG. MBD1 may also interact with histone methyltransferase SETDB1 to repress transcription.

Product Categories/Family for anti-MBD1 antibody

Epigenetics; MethyltransferaseAntibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 323462147
• NCBI GeneID: 4152
• NCBI Accession #: NP_001191065.1
• NCBI GenBank Nucleotide #: NM_001204136.1
• UniProt Accession #: Q9UIS9; O15248; O95241; Q7Z7B5; Q8N4W4; Q9UNZ6; A4UTZ0; B4DXJ5; E9PEC5; K7ELI2; K7EQZ4; K7ESN0
• Molecular Weight: 72,126 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 1 isoform 6
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 1
• NCBI Official Symbol: MBD1
• NCBI Official Synonym Symbols: RFT; PCM1; CXXC3
• NCBI Protein Information: methyl-CpG-binding domain protein 1
• UniProt Protein Name: Methyl-CpG-binding domain protein 1
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD1
• UniProt Synonym Gene Names: CXXC3; PCM1
• UniProt Entry Name: MBD1_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

MBD1: Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: heterochromatin; nuclear matrix; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; methyl-CpG binding; zinc ion binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent