Western Blot (WB)
(Caspase-14 mAb tested by Western blot. Caspase-14 detection by Western blot. The analysis was performed using 10 ug of mouse spleen cell extract and Caspase-14 mAb at a 2 ug/ml dilution.)
Rabbit anti-Human, Mouse Caspase-14 Monoclonal Antibody | anti-Casp14 antibody
Caspase-14 antibody (mAb)(Clone 70A1426)
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Western Blot (WB)
(Caspase-14 mAb tested by Western blot. Caspase-14 detection by Western blot. The analysis was performed using 10 ug of mouse spleen cell extract and Caspase-14 mAb at a 2 ug/ml dilution.)
Western Blot (WB)
(Caspase-14 mAb tested by Western blot. Caspase-14 detection by Western blot. The analysis was performed using 10 ug of mouse spleen cell extract and Caspase-14 mAb at a 2 ug/ml dilution.)
Background: Caspase-14 (CASP14 or apoptosis-related cysteine peptidase 14) is thought to be a non-apoptotic caspase involved in epidermal differentiation. Caspase-14 has been shown to be processed and activated by Caspase-8 and Caspase 10 in vitro, and also by TNF-Related Apoptosis-Inducing Ligand (TRAIL) in vivo.
NCBI and Uniprot Product Information
Uniprot Description
CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family.
Protein type: EC 3.4.22.-; Protease
Cellular Component: keratin filament; cytoplasm; nucleus
Molecular Function: peptidase activity; hydrolase activity; cysteine-type peptidase activity
Biological Process: caspase activation; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; cell differentiation; proteolysis
Research Articles on Casp14
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Product Notes
The Casp14 casp14 (Catalog #AAA388688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-14 antibody (mAb)(Clone 70A1426) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the Casp14 casp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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