Mouse anti-Human LSM8 Monoclonal Antibody | anti-LSM8 antibody

Mouse monoclonal antibody Anti-Human LSM8

Cat. Num. AAA120174

• Gene Names: LSM8; LSM8; NAA38
• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry
• Synonyms: LSM8; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human LSM8; Homo sapiens LSM8 homolog; U6 small nuclear RNA associated (S. cerevisiae) (LSM8); YJR022W; anti-LSM8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2527C6a
• Form/Format: Mouse monoclonal anti-human LSM8 antibody in PBS (3.0 mM KCI, 1.5 mM KH₂PO₄, 140 mM NaCl, 8.0 mM Na₂HPO₄ (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN₃)
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 12520

• Applicable Applications for anti-LSM8 antibody: Dot Blot (DB), Immunocytochemistry (ICC)
• Application Notes: ICC: 2-100 ug/ml
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: this antibody solutions contains sodium azide (NaN₃) as a preservative. There is a potential hazard NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Preparation and Storage: Store at 2-8°C for up to one year.; We recommend storing at -20°C for long-term storage.; Avoid repeat freezing and thawing cycles.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-LSM8 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LSM8 mAb. [Lot No.2527C6a-1])

Related Product Information for anti-LSM8 antibody

This gene is a member of the LSm family and encodes a protein with a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. The protein partners with six paralogs to form a heteroheptameric ring which transiently binds RNAs and is involved in the general maturation of RNA in the nucleus. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 284172355
• NCBI GeneID: 51691
• NCBI Accession #: NP_057284.1
• NCBI GenBank Nucleotide #: NM_016200
• NCBI Official Full Name: Homo sapiens LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae) (LSM8), mRNA
• NCBI Official Synonym Full Names: LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• NCBI Official Symbol: LSM8
• NCBI Official Synonym Symbols: LSM8; NAA38
• NCBI Protein Information: LSM8 homolog, U6 small nuclear RNA associated; MAK31-like protein; N-alpha-acetyltransferase 38, NatC auxiliary subunit; U6 snRNA-associated Sm-like protein LSm8
• UniProt Protein Name: U6 snRNA-associated Sm-like protein LSm8
• Protein Family: Sm-like protein
• UniProt Gene Name: LSM8
• UniProt Entry Name: LSM8_HUMAN

NCBI Description

This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus. [provided by RefSeq, Jan 2010]

Uniprot Description

LSM8: Binds specifically to the 3'-terminal U-tract of U6 snRNA. Belongs to the snRNP Sm proteins family.

Protein type: RNA splicing

Chromosomal Location of Human Ortholog: 7q31.31

Cellular Component: spliceosome; U4/U6 x U5 tri-snRNP complex

Molecular Function: protein binding; U6 snRNA 3'-end binding; U6 snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome