Mouse anti-Human DDX3X Monoclonal Antibody | anti-DDX3X antibody

Mouse monoclonal antibody Anti-Human DDX3X

Cat. Num. AAA120212

• Gene Names: DDX3X; DBX; DDX3; HLP2; DDX14; CAP-Rf
• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot
• Synonyms: DDX3X; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human DDX3X; Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 3; X-linked (DDX3X); DBX; DDX3; HLP2; DDX14; anti-DDX3X antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2253C5a
• Form/Format: Mouse monoclonal anti-human DDX3X antibody in PBS (3.0 mM KCl, 1.5 mM KH PO , 140 mM NaCl, 8.0 mM Na HPO (pH 7.4)) containing 1% bovine serum albumin (BSA)and 0.05% sodium azide (NaN ).
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 5519

• Applicable Applications for anti-DDX3X antibody: Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: WB: 0.2-2 ug/ml ; IP: 100-500 ug/sample ; ICC: 2-100 ug/ml
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Immunogen: Recombinant
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN3) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Preparation and Storage: Store at 2-8 degree C for up to 6 months.; We recommend storing at –20 degree C for long-term storage for up to one year.; Avoid repeat freezing and thawing cycles.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-DDX3X monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of DDX3X by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2253C5a-1]Predicted molecular weight: 73 kDa)

Immunoprecipitation (IP)

(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DDX3X mAb. [Lot No. 2253C5a-1]Predicted molecular weight: 73 kDa)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX3X mAb. [Lot No. 2253C5a-1])

Related Product Information for anti-DDX3X antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 698980011
• NCBI GeneID: 1654
• NCBI Accession #: NP_001347.3
• NCBI GenBank Nucleotide #: NM_001356
• NCBI Official Full Name: Homo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked (DDX3X), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked
• NCBI Official Symbol: DDX3X
• NCBI Official Synonym Symbols: DBX; DDX3; HLP2; DDX14; CAP-Rf
• NCBI Protein Information: ATP-dependent RNA helicase DDX3X; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD/H box-3; helicase-like protein 2
• UniProt Protein Name: ATP-dependent RNA helicase DDX3X
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX3X
• UniProt Entry Name: DDX3X_HUMAN

NCBI Description

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

DDX3: a multifunctional DEAD box family RNA helicase with diverse cellular functions. DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), and are involved in several steps of gene expression, such as transcription, maturation of nuclear and mitochondrial mRNA, mRNA export, translation initiation, and ribosome and spliceosome assembly. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1. DDX3 is required for hepatitis C virus (HCV) RNA replication and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC). May function as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC, and aberrant subcellular localization occurs in many squamous cell carcinomas. Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression. Associates with eIF4F to promote translation of selected mRNAs. Is phosphorylated by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation may cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis. Involved in TBK1 and IKBKE-dependent IRF3 activation leading to IFN-beta induction. Involved in regulation of apoptosis. May be required for activation of the intrinsic but inhibit activation of the extrinsic apoptotic pathway. Regulated by the cell cycle. Maximally expressed in the cytoplasm during G1/S phase and decreased expression during G2/M phase. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Associates with the outer side of nuclear pore complexes (NPC) and mitochondrial outer membranes. Shuttles between the nucleus and the cytoplasm in an exportin1-dependent manner. Associates with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G0 phase and in the cytoplasm during G1/S phase. Belongs to the DEAD box helicase family, DDX3 subfamily. Two isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Helicase; Spliceosome; Cell cycle regulation; EC 3.6.4.13; Translation initiation

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: eukaryotic translation initiation factor 3 complex; mitochondrial outer membrane; stress granule; cytoplasm; nuclear speck; nucleus

Molecular Function: GTPase activity; CTPase activity; RNA binding; ATPase activity; transcription factor binding; poly(A) binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; translation initiation factor binding; eukaryotic initiation factor 4E binding; nucleoside-triphosphatase activity; mRNA 5'-UTR binding; ATP binding; ATP-dependent RNA helicase activity; ribosomal small subunit binding

Biological Process: stress granule assembly; positive regulation of viral genome replication; viral reproduction; transcription, DNA-dependent; positive regulation of translation; positive regulation of apoptosis; response to virus; positive regulation of caspase activity; negative regulation of caspase activity; positive regulation of cell growth; DNA duplex unwinding; chromosome segregation; positive regulation of translational initiation; induction of apoptosis via death domain receptors; negative regulation of translation; RNA secondary structure unwinding; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of protein complex assembly; mature ribosome assembly; negative regulation of apoptosis

Disease: Mental Retardation, X-linked 102