Quality Control
(Western blot analysis of immunized recombinant protein, using anti-LRRFIP2 monoclonal antibody. )
Mouse anti-Human LRRFIP2 Monoclonal Antibody | anti-LRRFIP2 antibody
Mouse monoclonal antibody Anti-Human LRRFIP2
Gene Names
LRRFIP2; HUFI-2
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry
Synonyms
LRRFIP2; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human LRRFIP2; Homo sapiens leucine rich repeat (in FLII) interacting protein 2; mRNA (cDNA clone MGC:61455 IMAGE:6146978); complete cds.; FLJ20248; anti-LRRFIP2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
377C3a
Form/Format
Mouse monoclonal anti -human LRRFIP2 antibody in PBS (3.0 mM KCI, 1.5mM KH2PO4 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3)
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
1728
Applicable Applications for anti-LRRFIP2 antibody
Dot Blot (DB), Immunocytochemistry (ICC)
Application Notes
ICC: 2-100ug/ml
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Disposal
This antibody solution contains sodium azide (NaN3) as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water .
Preparation and Storage
Store at 2-8°C for up to a year. We recommended storing at -20°C for long term storage. Avoid repeat freezing and thawing cycles.
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-LRRFIP2 monoclonal antibody. )
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-LRRFIP2 monoclonal antibody. )
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Immunocytochemistry (ICC)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LRRFIP2 mAb. [LotNo. 377C3a-1])
Immunocytochemistry (ICC)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-LRRFIP2 mAb. [LotNo. 377C3a-1])
Related Product Information for anti-LRRFIP2 antibody
May function as activator of the canonical Wnt signaling pathway, in association with DVL3, upstream of CTNNB1/beta-catenin.[UniProtKB Function]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens leucine rich repeat (in FLII) interacting protein 2, mRNA
NCBI Official Synonym Full Names
leucine rich repeat (in FLII) interacting protein 2
NCBI Official Symbol
LRRFIP2
NCBI Official Synonym Symbols
HUFI-2
NCBI Protein Information
leucine-rich repeat flightless-interacting protein 2; LRR FLII-interacting protein 2
UniProt Protein Name
Leucine-rich repeat flightless-interacting protein 2
Protein Family
UniProt Gene Name
LRRFIP2
UniProt Entry Name
LRRF2_HUMAN
Uniprot Description
LRRFIP2: May function as activator of the canonical Wnt signaling pathway, in association with DVL3, upstream of CTNNB1/beta- catenin. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding. Belongs to the LRRFIP family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 3p22.2
Molecular Function: protein binding; LRR domain binding
Biological Process: Wnt receptor signaling pathway
Research Articles on LRRFIP2
1. FATylation of LRRFIP2 occurs on two distinct sites, each being modified by a single FAT10 moiety.
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Product Notes
The LRRFIP2 lrrfip2 (Catalog #AAA120549) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human LRRFIP2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LRRFIP2 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC). ICC: 2-100ug/ml. Researchers should empirically determine the suitability of the LRRFIP2 lrrfip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRRFIP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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